Detailed information of TA system TA073417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 594831..595407
• Replicon: chromosome
• Accession: NZ_CP019889
• Organism: Enterobacter hormaechei strain FRM

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: B1023_RS02945
• Protein ID: WP_015572580.1
• Coordinates: 594831..595118 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: B1023_RS02950
• Protein ID: WP_017694570.1
• Coordinates: 595105..595407 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1023_RS02920	589966..590643	+	678	WP_118379265.1	winged helix-turn-helix domain-containing protein	-
B1023_RS02925	590793..591263	+	471	WP_015572576.1	MarR family transcriptional regulator	-
B1023_RS02930	591260..592327	+	1068	WP_026080654.1	HlyD family secretion protein	-
B1023_RS02935	592317..593393	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
B1023_RS02940	593390..594661	-	1272	WP_045348387.1	DUF445 domain-containing protein	-
B1023_RS02945	594831..595118	+	288	WP_015572580.1	BrnT family toxin	Toxin
B1023_RS02950	595105..595407	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
B1023_RS02955	595436..596074	-	639	WP_045348388.1	LysE family translocator	-
B1023_RS02960	596113..596865	-	753	WP_063137153.1	AraC family transcriptional regulator	-
B1023_RS02965	597019..598389	+	1371	WP_015572583.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
B1023_RS02970	598569..599114	-	546	WP_006810254.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T73417 WP_015572580.1 NZ_CP019889:594831-595118 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T73417 NZ_CP019889:594831-595118 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAGTGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAGGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT73417 WP_017694570.1 NZ_CP019889:595105-595407 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT73417 NZ_CP019889:595105-595407 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA