Detailed information of TA system TA073416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 285656..286252
• Replicon: chromosome
• Accession: NZ_CP019889
• Organism: Enterobacter hormaechei strain FRM

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: B1023_RS01430
• Protein ID: WP_023295756.1
• Coordinates: 285950..286252 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: B1023_RS01425
• Protein ID: WP_023295755.1
• Coordinates: 285656..285943 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1023_RS01420	284028..285659	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
B1023_RS01425	285656..285943	-	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
B1023_RS01430	285950..286252	-	303	WP_023295756.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1023_RS01435	286450..287322	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
B1023_RS01440	287323..287595	-	273	WP_017382612.1	DUF3811 domain-containing protein	-
B1023_RS01445	287646..288590	-	945	WP_015570166.1	ketopantoate/pantoate/pantothenate transporter PanS	-
B1023_RS01450	288684..290033	-	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11441.24 Da
• Isoelectric Point: 10.1042

>T73416 WP_023295756.1 NZ_CP019889:c286252-285950 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T73416 NZ_CP019889:c286252-285950 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
CTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCTGTGGGCGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT73416 WP_023295755.1 NZ_CP019889:c285943-285656 [Enterobacter hormaechei]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT73416 NZ_CP019889:c285943-285656 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA