Detailed information of TA system TA073413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 27835..28441
• Replicon: plasmid pKNA02
• Accession: NZ_CP019877
• Organism: Komagataeibacter nataicola strain RZS01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: B0W47_RS16750
• Protein ID: WP_078528458.1
• Coordinates: 28262..28441 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B0W47_RS16745
• Protein ID: WP_078528456.1
• Coordinates: 27835..28251 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W47_RS16715	23265..23597	-	333	WP_167370270.1	transposase	-
B0W47_RS16720	23739..25388	-	1650	WP_078528507.1	helix-turn-helix domain-containing protein	-
B0W47_RS16725	25779..26549	-	771	WP_078528510.1	StbB	-
B0W47_RS16730	26644..26868	+	225	WP_078528513.1	helix-turn-helix domain-containing protein	-
B0W47_RS18585	26906..27082	+	177	WP_167370271.1	hypothetical protein	-
B0W47_RS16740	27504..27758	-	255	WP_078528516.1	hypothetical protein	-
B0W47_RS16745	27835..28251	-	417	WP_078528456.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B0W47_RS16750	28262..28441	-	180	WP_078528458.1	type II toxin-antitoxin system HicA family toxin	Toxin
B0W47_RS16760	29203..30138	+	936	WP_078528519.1	LysR family transcriptional regulator	-
B0W47_RS16765	30194..32539	+	2346	WP_078528522.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38682	38682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6478.67 Da
• Isoelectric Point: 11.7334

>T73413 WP_078528458.1 NZ_CP019877:c28441-28262 [Komagataeibacter nataicola]
MKSANLIRELEKAGWKLDRVRGSHNVFKHPSRPGIVVVPHPKKDLGTGLVAAIRKQAGL

Nucleotide

• Length: 180 bp

>T73413 NZ_CP019877:c28441-28262 [Komagataeibacter nataicola]
ATGAAGAGTGCCAATCTGATCCGGGAGCTGGAAAAAGCAGGCTGGAAGCTGGATCGCGTGCGCGGTTCACACAACGTCTT
CAAACATCCGTCGCGACCTGGGATCGTGGTCGTCCCTCATCCCAAAAAGGATCTGGGCACCGGTCTGGTTGCCGCCATCC
GCAAACAGGCGGGTCTCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14890.73 Da
• Isoelectric Point: 4.2253

>AT73413 WP_078528456.1 NZ_CP019877:c28251-27835 [Komagataeibacter nataicola]
MRYPVVIERGSETTAFGVVFPDLPGCFSAGDTLDEAISNAEEAAAAWIDATLDAGEAVPFPSTLDAIQNNPDYEGWTVGF
VTIDPAVLDDRVERANISLPRRVLERLDALARSSHESRSGMIAAMTLQARLTPVVRSK

Nucleotide

• Length: 417 bp

>AT73413 NZ_CP019877:c28251-27835 [Komagataeibacter nataicola]
ATGCGCTATCCGGTTGTTATCGAACGGGGCAGCGAGACAACGGCATTTGGTGTTGTCTTTCCTGACCTTCCCGGCTGCTT
TTCGGCAGGCGACACGCTCGATGAAGCCATTTCCAATGCGGAAGAGGCCGCTGCGGCATGGATCGATGCCACGCTGGATG
CCGGAGAGGCTGTGCCTTTTCCCAGCACGCTCGACGCCATCCAGAACAACCCGGATTATGAGGGCTGGACAGTGGGCTTT
GTTACCATTGATCCGGCCGTTCTTGACGACCGGGTGGAACGGGCGAACATCTCCCTTCCCCGGCGTGTTCTGGAGCGGCT
GGATGCGCTTGCCAGATCATCCCATGAGAGCCGTTCCGGCATGATTGCGGCCATGACCCTGCAGGCAAGACTTACACCGG
TGGTCAGAAGCAAATGA