Detailed information of TA system TA073412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 10101..10666
• Replicon: plasmid pKNA02
• Accession: NZ_CP019877
• Organism: Komagataeibacter nataicola strain RZS01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B0W47_RS16635
• Protein ID: WP_078528469.1
• Coordinates: 10101..10382 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B0W47_RS16640
• Protein ID: WP_078528472.1
• Coordinates: 10379..10666 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W47_RS16610	6276..7307	+	1032	WP_078528463.1	Ldh family oxidoreductase	-
B0W47_RS16615	7443..8070	-	628	Protein_6	transposase	-
B0W47_RS18580	8069..8239	-	171	Protein_7	IS110 family transposase	-
B0W47_RS16620	8501..8914	+	414	WP_078528466.1	recombinase family protein	-
B0W47_RS16630	9743..9880	+	138	Protein_10	Hin recombinase	-
B0W47_RS16635	10101..10382	-	282	WP_078528469.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0W47_RS16640	10379..10666	-	288	WP_078528472.1	ribbon-helix-helix domain-containing protein	Antitoxin
B0W47_RS16645	11485..12390	-	906	WP_078528475.1	replication protein RepA	-
B0W47_RS16650	12592..12846	-	255	WP_078528478.1	ribbon-helix-helix domain-containing protein	-
B0W47_RS16655	12876..13526	-	651	WP_078528481.1	AAA family ATPase	-
B0W47_RS16660	13665..14300	-	636	WP_078528484.1	tyrosine-type recombinase/integrase	-
B0W47_RS16665	14297..14593	-	297	WP_078528487.1	WGR domain-containing protein	-
B0W47_RS16670	14904..15431	+	528	Protein_18	phage integrase N-terminal SAM-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38682	38682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-48)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10798.30 Da
• Isoelectric Point: 6.7246

>T73412 WP_078528469.1 NZ_CP019877:c10382-10101 [Komagataeibacter nataicola]
VKYEWTAKAVSDLQTLAANFDHPSGAARVMRRLTAAPDVLIQFPRMGTLLPVFSPREVRRFIVDDYEIRYEVTADALFIT
DVFHTRQDRETFH

Nucleotide

• Length: 282 bp

>T73412 NZ_CP019877:c10382-10101 [Komagataeibacter nataicola]
GTGAAATATGAATGGACGGCCAAGGCCGTATCTGATCTGCAAACGCTAGCCGCAAATTTTGACCACCCGAGCGGCGCCGC
GCGTGTTATGCGGCGGCTGACGGCAGCCCCGGACGTTCTGATCCAGTTCCCTCGGATGGGTACGTTGCTTCCGGTCTTCT
CACCCCGTGAGGTCCGGCGCTTCATCGTGGATGACTATGAAATTCGCTATGAGGTCACGGCGGATGCCCTGTTCATCACC
GACGTTTTCCATACGAGGCAGGACCGCGAGACGTTTCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10516.93 Da
• Isoelectric Point: 4.8660

>AT73412 WP_078528472.1 NZ_CP019877:c10666-10379 [Komagataeibacter nataicola]
MSEKLSAGKQVVSVQMPADLVDAMDRYGEAIERPRSWVMREAVASYLAYEAEKDRVTRRALADVDAGRTISAEAVKAWAD
SLDQANPLPLPEPKR

Nucleotide

• Length: 288 bp

>AT73412 NZ_CP019877:c10666-10379 [Komagataeibacter nataicola]
ATGAGCGAGAAACTTTCAGCCGGAAAGCAGGTGGTCAGTGTGCAGATGCCTGCCGACCTGGTGGATGCGATGGATCGATA
TGGGGAAGCCATCGAGCGGCCCAGGTCATGGGTGATGCGGGAAGCCGTGGCGTCTTACCTTGCCTATGAGGCAGAGAAAG
ACCGGGTGACGCGCCGTGCCCTGGCCGATGTAGATGCTGGCCGGACGATCAGTGCTGAGGCCGTCAAGGCATGGGCAGAC
AGTCTCGACCAAGCTAACCCGCTGCCGCTGCCGGAACCCAAGCGGTGA