Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 76417..76991 Replicon plasmid pB13-200C
Accession NZ_CP019874
Organism Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag XJ28_RS31795 Protein ID WP_003344395.1
Coordinates 76614..76991 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48BC4
Locus tag XJ28_RS31790 Protein ID WP_004644067.1
Coordinates 76417..76617 (+) Length 67 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XJ28_RS31770 71930..72493 - 564 WP_010214078.1 hypothetical protein -
XJ28_RS31775 72514..72936 - 423 WP_007247232.1 hypothetical protein -
XJ28_RS31780 72964..75252 - 2289 WP_105224219.1 type IV secretory system conjugative DNA transfer family protein -
XJ28_RS31785 75239..76324 - 1086 WP_060404100.1 thioredoxin fold domain-containing protein -
XJ28_RS31790 76417..76617 + 201 WP_004644067.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
XJ28_RS31795 76614..76991 + 378 WP_003344395.1 PIN domain-containing protein Toxin
XJ28_RS31800 76981..78183 - 1203 WP_105224220.1 conjugal transfer protein -
XJ28_RS31805 78276..78923 - 648 WP_007247237.1 plasmid IncI1-type surface exclusion protein ExcA -
XJ28_RS31810 78972..81113 - 2142 WP_007247238.1 DotA/TraY family protein -
XJ28_RS31815 81142..81744 - 603 WP_007247239.1 conjugal transfer protein TraX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..82965 82965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-113)

Antitoxin

(1-43)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T73409 WP_003344395.1 NZ_CP019874:76614-76991 [Pseudomonas syringae pv. tomato]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T73409 NZ_CP019874:76614-76991 [Pseudomonas syringae pv. tomato]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCACCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAC
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGTCGCATGGCCGTGTCTTACCAGCCTCCTACTCACTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7445.66 Da        Isoelectric Point: 7.0102

>AT73409 WP_004644067.1 NZ_CP019874:76417-76617 [Pseudomonas syringae pv. tomato]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPTMEDIARRHEKAL

Download         Length: 201 bp

>AT73409 NZ_CP019874:76417-76617 [Pseudomonas syringae pv. tomato]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCAGCTAAACGATTGATGGCCTTAGGGGCCACACTGCCAA
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2


Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IKB9

References