Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 62106..62779 Replicon plasmid pB13-200C
Accession NZ_CP019874
Organism Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)


Gene name vapC Uniprot ID A0A4P6QS82
Locus tag XJ28_RS31705 Protein ID WP_007247669.1
Coordinates 62106..62525 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag XJ28_RS31710 Protein ID WP_003381346.1
Coordinates 62522..62779 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XJ28_RS31680 57116..58150 - 1035 WP_010212085.1 sulfotransferase -
XJ28_RS31685 58168..59088 - 921 WP_007247581.1 TauD/TfdA family dioxygenase -
XJ28_RS31690 59234..59881 - 648 WP_007247582.1 histidine phosphatase family protein -
XJ28_RS31695 60223..61158 - 936 WP_007247583.1 type III effector avrD1 -
XJ28_RS31700 61433..62065 - 633 WP_054091164.1 recombinase family protein -
XJ28_RS31705 62106..62525 - 420 WP_007247669.1 type II toxin-antitoxin system VapC family toxin Toxin
XJ28_RS31710 62522..62779 - 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
XJ28_RS31720 63494..65049 + 1556 WP_086003402.1 IS3-like element IS1240 family transposase -
XJ28_RS31730 66066..66764 - 699 WP_007247667.1 GNAT family N-acetyltransferase -
XJ28_RS31735 67005..67292 - 288 WP_010209640.1 HU family DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..82965 82965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15156.46 Da        Isoelectric Point: 4.7010

>T73408 WP_007247669.1 NZ_CP019874:c62525-62106 [Pseudomonas syringae pv. tomato]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYMSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T73408 NZ_CP019874:c62525-62106 [Pseudomonas syringae pv. tomato]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATATGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT73408 WP_003381346.1 NZ_CP019874:c62779-62522 [Pseudomonas syringae pv. tomato]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT73408 NZ_CP019874:c62779-62522 [Pseudomonas syringae pv. tomato]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCCATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P6QS82

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References