Detailed information of TA system TA073407

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 12199..12810
• Replicon: plasmid pB13-200B
• Accession: NZ_CP019873
• Organism: Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: XJ28_RS30830
• Protein ID: WP_105224178.1
• Coordinates: 12199..12549 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: XJ28_RS30835
• Protein ID: WP_105224179.1
• Coordinates: 12562..12810 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XJ28_RS30825	10349..11779	+	1431	WP_105223910.1	IS1182 family transposase	-
XJ28_RS30830	12199..12549	-	351	WP_105224178.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XJ28_RS30835	12562..12810	-	249	WP_105224179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
XJ28_RS30840	13141..14100	+	960	WP_105224213.1	tyrosine-type recombinase/integrase	-
XJ28_RS30845	14131..15114	-	984	Protein_10	type III effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93873	93873
	-	flank	IS/Tn	-	-	10349..11779	1430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12896.69 Da
• Isoelectric Point: 4.1953

>T73407 WP_105224178.1 NZ_CP019873:c12549-12199 [Pseudomonas syringae pv. tomato]
MTTFALRFTEVAQQSLEDQVEHLAVTQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGVTDIAVLLVLGGKQSVEQALIRYCLLQTM

Nucleotide

• Length: 351 bp

>T73407 NZ_CP019873:c12549-12199 [Pseudomonas syringae pv. tomato]
ATGACTACATTTGCACTGCGCTTTACCGAGGTTGCGCAGCAAAGCCTTGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGTATAGATTCCCTGATCGATGCCATCCAGGACAAGCTCCTGTCCACGCCGTTGGGCT
ACCCGGTTAGTCCTCAATTGAGTGAGCTGGGCGTGTTGCACTATCGTGAGTTGAACACTGACGGATACCGAATCTTTTAC
GAGGTGATGGATTCGGATGGTGTTACCGACATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTTGAACAAGCCCT
GATCCGCTACTGCCTGCTTCAGACTATGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9401.73 Da
• Isoelectric Point: 9.5019

>AT73407 WP_105224179.1 NZ_CP019873:c12810-12562 [Pseudomonas syringae pv. tomato]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSPDELKARLSRRFD
HK

Nucleotide

• Length: 249 bp

>AT73407 NZ_CP019873:c12810-12562 [Pseudomonas syringae pv. tomato]
ATGCGAGTTGAAACAATAAGTTATCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCACTGGTCGTCACGCAAAA
CGGTGTCCCGGCTTATGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAATCCATCGCCTTGGTGAAGCTCCTGG
CAATCAGTTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCCCTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAT
CACAAGTAA