Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 30023..30690 Replicon plasmid pB13-200A
Accession NZ_CP019872
Organism Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)


Gene name vapC Uniprot ID A0A0Q0GBP3
Locus tag XJ28_RS30280 Protein ID WP_002556038.1
Coordinates 30274..30690 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID E7PUB4
Locus tag XJ28_RS30275 Protein ID WP_002556039.1
Coordinates 30023..30277 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XJ28_RS30255 25431..26447 - 1017 WP_057425386.1 ABC transporter permease -
XJ28_RS30260 26540..28120 - 1581 WP_105224134.1 ABC transporter substrate-binding protein -
XJ28_RS30265 28239..28778 - 540 WP_004642348.1 D-alanyl-D-alanine dipeptidase -
XJ28_RS30270 28816..29676 - 861 WP_004668209.1 MurR/RpiR family transcriptional regulator -
XJ28_RS30275 30023..30277 + 255 WP_002556039.1 plasmid stabilization protein Antitoxin
XJ28_RS30280 30274..30690 + 417 WP_002556038.1 type II toxin-antitoxin system VapC family toxin Toxin
XJ28_RS30285 30952..32907 - 1956 WP_105224135.1 relaxase/mobilization nuclease domain-containing protein -
XJ28_RS30290 32918..33247 - 330 WP_060403683.1 ribbon-helix-helix protein, CopG family -
XJ28_RS30295 33682..33960 + 279 WP_105224136.1 mobilization protein -
XJ28_RS30300 34034..34234 - 201 WP_105224137.1 hypothetical protein -
XJ28_RS30305 34303..35139 - 837 WP_105224138.1 DUF945 domain-containing protein -
XJ28_RS30310 35185..35562 - 378 WP_105224139.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..125801 125801


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14865.14 Da        Isoelectric Point: 4.6579

>T73406 WP_002556038.1 NZ_CP019872:30274-30690 [Pseudomonas syringae pv. tomato]
MIVLDTNVVSEAMKPESHLAVRAWLNDQAAETLYLSSVTLAELLFGIGALPAGKRKDMLAQALDGLMGLFRDRVLPFDID
AARRYADLAVTAKTCGRGFPTPDGYIAAIAASRDFIVASRDTAPYEAVGVPVINPWEV

Download         Length: 417 bp

>T73406 NZ_CP019872:30274-30690 [Pseudomonas syringae pv. tomato]
ATGATCGTCCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGTCCCACTTGGCCGTGCGGGCCTGGCTGAACGA
TCAAGCCGCCGAAACGCTATACCTGTCCAGCGTGACGCTGGCCGAGCTGCTGTTTGGCATCGGGGCGCTCCCGGCCGGCA
AGCGCAAGGATATGCTGGCGCAGGCCCTTGACGGTCTAATGGGGCTGTTCAGGGATCGGGTGCTGCCATTCGATATCGAT
GCAGCACGGCGCTATGCCGATCTGGCCGTGACGGCAAAAACCTGCGGGCGAGGATTCCCGACACCTGACGGCTACATCGC
AGCGATTGCGGCATCGCGAGATTTCATCGTGGCGTCACGCGACACTGCACCCTATGAGGCGGTCGGTGTACCCGTCATCA
ATCCGTGGGAAGTGTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9442.84 Da        Isoelectric Point: 7.3219

>AT73406 WP_002556039.1 NZ_CP019872:30023-30277 [Pseudomonas syringae pv. tomato]
MAMLTVRNLPDDVHRALRVRAAQHGHSTEAEVREILAIAVKPETRVRLGEALAALGRKIGLTNEDFEVFNQVRDKTPAEP
LRFE

Download         Length: 255 bp

>AT73406 NZ_CP019872:30023-30277 [Pseudomonas syringae pv. tomato]
ATGGCAATGCTGACAGTACGGAACTTGCCCGACGATGTACACCGCGCGCTACGAGTGCGGGCCGCCCAACACGGGCACAG
CACCGAGGCCGAAGTCCGCGAGATTCTGGCGATCGCAGTCAAGCCGGAGACGCGCGTTCGCCTAGGTGAAGCCTTGGCGG
CATTGGGTCGCAAGATCGGCCTGACGAACGAAGATTTCGAGGTCTTCAACCAAGTGCGAGACAAGACGCCGGCCGAGCCG
CTGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0GBP3

Antitoxin

Source ID Structure
AlphaFold DB A0A2G9L5L9

References