Detailed information of TA system TA073403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2262198..2262711
• Replicon: chromosome
• Accession: NZ_CP019871
• Organism: Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: XJ28_RS11390
• Protein ID: WP_007245247.1
• Coordinates: 2262198..2262482 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6UR83
• Locus tag: XJ28_RS11395
• Protein ID: WP_005740683.1
• Coordinates: 2262472..2262711 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XJ28_RS11370	2257607..2258092	+	486	WP_007245243.1	DUF2269 domain-containing protein	-
XJ28_RS11375	2258344..2260272	+	1929	WP_007245244.1	methyl-accepting chemotaxis protein	-
XJ28_RS11380	2260301..2261233	-	933	WP_007245245.1	DMT family transporter	-
XJ28_RS11385	2261308..2262162	+	855	WP_007245246.1	LysR family transcriptional regulator	-
XJ28_RS11390	2262198..2262482	-	285	WP_007245247.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XJ28_RS11395	2262472..2262711	-	240	WP_005740683.1	ribbon-helix-helix protein, CopG family	Antitoxin
XJ28_RS11400	2262880..2263896	-	1017	WP_003376162.1	1-aminocyclopropane-1-carboxylate deaminase	-
XJ28_RS11405	2264062..2264568	+	507	WP_007245249.1	winged helix-turn-helix transcriptional regulator	-
XJ28_RS11415	2264716..2265624	+	909	WP_010203521.1	DUF808 domain-containing protein	-
XJ28_RS11420	2265751..2266536	-	786	WP_007245250.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10828.58 Da
• Isoelectric Point: 10.0884

>T73403 WP_007245247.1 NZ_CP019871:c2262482-2262198 [Pseudomonas syringae pv. tomato]
MQVEWLKTALKNLDDEAAYISLENPAAAVAFVEAIQISVKQLASFPALGREGRIAGTREWPLPDWPYLIPYRIRNGRLQV
LRIFHTRRQSPLVW

Nucleotide

• Length: 285 bp

>T73403 NZ_CP019871:c2262482-2262198 [Pseudomonas syringae pv. tomato]
ATGCAGGTTGAATGGCTAAAAACAGCATTAAAAAACCTGGATGATGAAGCCGCCTACATCTCGCTAGAAAATCCGGCTGC
TGCGGTTGCTTTCGTAGAAGCAATACAAATAAGCGTAAAGCAGCTCGCAAGCTTTCCGGCCCTAGGCCGAGAGGGACGAA
TTGCTGGCACCAGAGAATGGCCTTTGCCAGACTGGCCTTACCTGATTCCATACCGCATCCGTAACGGCCGATTACAGGTT
CTGCGGATTTTCCACACGAGAAGGCAGTCGCCGTTGGTGTGGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8753.90 Da
• Isoelectric Point: 4.5822

>AT73403 WP_005740683.1 NZ_CP019871:c2262711-2262472 [Pseudomonas syringae pv. tomato]
MSVMSLRLPDEMADTLAHLAKATGRSKSFLALNALREYLTREAWQIAEIQRAIEEADAGEFASEEDVKAVMNKWANNAG

Nucleotide

• Length: 240 bp

>AT73403 NZ_CP019871:c2262711-2262472 [Pseudomonas syringae pv. tomato]
ATGTCCGTTATGTCTCTACGCCTGCCGGACGAGATGGCAGATACCCTTGCACACCTCGCCAAAGCGACCGGCCGCAGCAA
GTCGTTTTTGGCCTTGAACGCATTACGTGAATACTTGACTCGCGAAGCCTGGCAGATTGCCGAAATTCAGCGTGCAATTG
AAGAAGCAGACGCGGGCGAGTTCGCAAGTGAAGAAGATGTAAAGGCAGTGATGAATAAATGGGCGAACAATGCAGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8M7Q4