Detailed information of TA system TA073400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 228467..229081
• Replicon: chromosome
• Accession: NZ_CP019871
• Organism: Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q88AZ2
• Locus tag: XJ28_RS01225
• Protein ID: WP_005768484.1
• Coordinates: 228731..229081 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: Q88AZ3
• Locus tag: XJ28_RS01220
• Protein ID: WP_007246542.1
• Coordinates: 228467..228718 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XJ28_RS01195	223533..223976	-	444	WP_172679536.1	ATP-dependent zinc protease	-
XJ28_RS01200	224026..224808	-	783	WP_010206181.1	EAL domain-containing protein	-
XJ28_RS01205	224976..225383	+	408	WP_007246541.1	RNA-binding protein S4	-
XJ28_RS01210	225544..226446	+	903	WP_005613652.1	Hsp33 family molecular chaperone HslO	-
XJ28_RS01215	226625..228169	+	1545	WP_005613653.1	phosphoenolpyruvate carboxykinase	-
XJ28_RS01220	228467..228718	+	252	WP_007246542.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
XJ28_RS01225	228731..229081	+	351	WP_005768484.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XJ28_RS01230	229383..229835	-	453	WP_007246543.1	hypothetical protein	-
XJ28_RS01235	230226..230840	-	615	WP_010206280.1	hypothetical protein	-
XJ28_RS01240	230937..231644	-	708	WP_059296115.1	hypothetical protein	-
XJ28_RS01245	231920..232270	+	351	Protein_230	phosphoenolpyruvate carboxykinase (ATP)	-
XJ28_RS01250	232310..232813	-	504	Protein_231	hypothetical protein	-
XJ28_RS01255	232876..233856	+	981	WP_105223850.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	232876..233856	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13110.05 Da
• Isoelectric Point: 4.3176

>T73400 WP_005768484.1 NZ_CP019871:228731-229081 [Pseudomonas syringae pv. tomato]
MNMFALRFTDVAQQSLEDQVEHLAVYQGFSPAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNADGYRIFY
EVRETDDINVIVIVLVLGGKQSVEQALIRYCLLQPI

Nucleotide

• Length: 351 bp

>T73400 NZ_CP019871:228731-229081 [Pseudomonas syringae pv. tomato]
ATGAATATGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCGGTCTATCA
GGGATTCTCTCCCGCTGCTCAGCGCATAGACACCCTGATTGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACGCTGACGGATACCGGATCTTTTAC
GAGGTGAGGGAGACAGATGATATTAATGTCATCGTCATAGTGCTCGTATTAGGTGGTAAACAAAGTGTTGAACAGGCACT
AATCCGCTATTGTCTGCTTCAGCCTATTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9474.83 Da
• Isoelectric Point: 9.5019

>AT73400 WP_007246542.1 NZ_CP019871:228467-228718 [Pseudomonas syringae pv. tomato]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSADELKARLSRRFA
HKE

Nucleotide

• Length: 252 bp

>AT73400 NZ_CP019871:228467-228718 [Pseudomonas syringae pv. tomato]
ATGCGAGTTGAAACAATAAGCTATTTGAAACGCAATGCAGCCGATCTGCCTCTCGATGAGCCACTGATTGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGTCGCGATGAGTCCATTGCCTTGGTGAAGCTCTTGG
CAATCAGTTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCTGCTGACGAACTTAAAGCCCGATTGAGCAGGAGGTTTGCC
CACAAAGAATAA

Structures

Toxin

• AlphaFold DB: Q88AZ2

Antitoxin

• AlphaFold DB: Q88AZ3