Detailed information of TA system TA073336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2497892..2498505
• Replicon: chromosome
• Accession: NZ_CP019856
• Organism: Pseudomonas azotoformans strain F77

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: B1R45_RS11585
• Protein ID: WP_078047964.1
• Coordinates: 2498254..2498505 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B1R45_RS11580
• Protein ID: WP_078047963.1
• Coordinates: 2497892..2498257 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1R45_RS11560	2493630..2494613	+	984	WP_078047959.1	ATP-dependent Clp protease ATP-binding subunit	-
B1R45_RS11565	2494651..2496006	+	1356	WP_078047960.1	MATE family efflux transporter	-
B1R45_RS11570	2495990..2496577	-	588	WP_078047961.1	TetR/AcrR family transcriptional regulator	-
B1R45_RS11575	2496719..2497891	+	1173	WP_078047962.1	MFS transporter	-
B1R45_RS11580	2497892..2498257	-	366	WP_078047963.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B1R45_RS11585	2498254..2498505	-	252	WP_078047964.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9488.02 Da
• Isoelectric Point: 10.0459

>T73336 WP_078047964.1 NZ_CP019856:c2498505-2498254 [Pseudomonas azotoformans]
VSKNEKLLAKLLNEHTAFTWPELVTLLRRLGYTQIEGAGSRVKFDIGDPSAMITLHKPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T73336 NZ_CP019856:c2498505-2498254 [Pseudomonas azotoformans]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAACGAGCACACGGCATTTACCTGGCCCGAACTCGTGACACTGCT
GCGCCGGCTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGCGTCAAATTCGACATCGGTGACCCCAGTGCAATGATTA
CCTTGCACAAGCCTCACCCGGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAGCAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13150.92 Da
• Isoelectric Point: 6.6267

>AT73336 WP_078047963.1 NZ_CP019856:c2498257-2497892 [Pseudomonas azotoformans]
VNNQLQHKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDGAFREAVDDYLATCQALGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHGNDPCPAVS

Nucleotide

• Length: 366 bp

>AT73336 NZ_CP019856:c2498257-2497892 [Pseudomonas azotoformans]
GTGAACAATCAACTGCAACACAAAGGCTACATCGGCTCTATCGAAGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAGAT
TCTGTTCATCAAGGCGCTGGTGAGCTACGAAGGAAAAACCGTCGCCGAGCTGGACGGCGCGTTCCGGGAGGCGGTGGACG
ACTACCTCGCCACCTGCCAGGCCCTGGGGCAAACACCGGAAAAACCCTGCAAGGGTTCATTCAACGTACGCGTCGGCCAT
GACTTGCACTTGGCGGCAGCCCTGGCGGCGACCCGCAAAAAAGTGACCCTCAATGACCTGACACGCCAGGCCTTGAATGA
GTTTTTACAACATCATGGCAACGACCCGTGCCCTGCGGTTAGCTGA