Detailed information of TA system TA073335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2038578..2039182
• Replicon: chromosome
• Accession: NZ_CP019856
• Organism: Pseudomonas azotoformans strain F77

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1U9PV57
• Locus tag: B1R45_RS09345
• Protein ID: WP_073859991.1
• Coordinates: 2038578..2038766 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B1R45_RS09350
• Protein ID: WP_078047607.1
• Coordinates: 2038778..2039182 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1R45_RS09320	2034268..2034987	+	720	WP_078047602.1	phosphonate C-P lyase system protein PhnL	-
B1R45_RS09325	2034977..2036122	+	1146	WP_078047603.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
B1R45_RS09330	2036122..2036694	+	573	WP_078047604.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
B1R45_RS09335	2036685..2037434	+	750	WP_078047605.1	phosphonate metabolism protein PhnP	-
B1R45_RS09340	2037517..2038395	-	879	WP_078047606.1	LysR family transcriptional regulator	-
B1R45_RS09345	2038578..2038766	+	189	WP_073859991.1	type II toxin-antitoxin system HicA family toxin	Toxin
B1R45_RS09350	2038778..2039182	+	405	WP_078047607.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B1R45_RS09355	2039576..2041105	+	1530	WP_078047608.1	M10 family metallopeptidase C-terminal domain-containing protein	-
B1R45_RS09360	2041348..2043081	+	1734	WP_078047609.1	M10 family metallopeptidase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7192.39 Da
• Isoelectric Point: 11.2000

>T73335 WP_073859991.1 NZ_CP019856:2038578-2038766 [Pseudomonas azotoformans]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYSPYTLPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T73335 NZ_CP019856:2038578-2038766 [Pseudomonas azotoformans]
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCAGGTTGGGTGCTGGATCGGGTCACGGGAAGTCATCATATTTT
TACCCATCGCTATAGCCCGTACACCCTTCCAGTGCCCCATCCAAAGAAGGACTTGCCGCTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14745.73 Da
• Isoelectric Point: 4.4776

>AT73335 WP_078047607.1 NZ_CP019856:2038778-2039182 [Pseudomonas azotoformans]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGTIPMPTSVAQYHAHEDYAGMGWGM
LELDVTPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT73335 NZ_CP019856:2038778-2039182 [Pseudomonas azotoformans]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACTGCCACCGGTATCCAGATCCCCGATATCCCAGGTGCAGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTACAACGCCGCCGTGGAAGTCGCGCACATCATGCTGCAAGAGATCGCCGCAG
AGGGCGGGACGATTCCGATGCCCACGTCGGTCGCCCAGTACCATGCCCACGAGGACTACGCCGGGATGGGCTGGGGTATG
TTGGAACTGGACGTCACGCCCTACCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCCGGTTATGTCATCCAGCGCAT
CGACCGCTATGTGCGAGAGCACAAGGTCAAAAGCCGCTCGTCATTCTTGGCGGACGCGGCATTGGAGAAGTTGGTGCGGT
CCTAG

Structures

Toxin

• AlphaFold DB: A0A1U9PV57