Detailed information of TA system TA073332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4207289..4207865
• Replicon: chromosome
• Accession: NZ_CP019839
• Organism: Enterobacter roggenkampii strain R11

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: B1H21_RS20280
• Protein ID: WP_063418479.1
• Coordinates: 4207578..4207865 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A156JBA1
• Locus tag: B1H21_RS20275
• Protein ID: WP_023293359.1
• Coordinates: 4207289..4207591 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H21_RS20250	4203396..4203941	+	546	WP_063418482.1	porin	-
B1H21_RS20255	4203941..4204261	+	321	WP_008501314.1	hypothetical protein	-
B1H21_RS20260	4204307..4205677	-	1371	WP_058654250.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
B1H21_RS20265	4205829..4206572	+	744	WP_063418481.1	AraC family transcriptional regulator	-
B1H21_RS20270	4206620..4207258	+	639	WP_063418480.1	LysE family translocator	-
B1H21_RS20275	4207289..4207591	-	303	WP_023293359.1	BrnA antitoxin family protein	Antitoxin
B1H21_RS20280	4207578..4207865	-	288	WP_063418479.1	BrnT family toxin	Toxin
B1H21_RS20285	4208034..4209305	+	1272	WP_063418478.1	DUF445 domain-containing protein	-
B1H21_RS20290	4209302..4210378	-	1077	WP_045286439.1	DUF2955 domain-containing protein	-
B1H21_RS20295	4210368..4211435	-	1068	WP_088728436.1	HlyD family secretion protein	-
B1H21_RS20300	4211432..4211902	-	471	WP_021240623.1	MarR family transcriptional regulator	-
B1H21_RS20305	4212048..4212743	-	696	WP_063418331.1	winged helix-turn-helix domain-containing protein	-
B1H21_RS20310	4212736..4212852	-	117	Protein_3917	amino acid-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11292.79 Da
• Isoelectric Point: 7.4007

>T73332 WP_063418479.1 NZ_CP019839:c4207865-4207578 [Enterobacter roggenkampii]
MPMEYEWDNNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T73332 NZ_CP019839:c4207865-4207578 [Enterobacter roggenkampii]
ATGCCAATGGAGTATGAGTGGGATAACAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGGGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTTTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11369.01 Da
• Isoelectric Point: 10.2483

>AT73332 WP_023293359.1 NZ_CP019839:c4207591-4207289 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT73332 NZ_CP019839:c4207591-4207289 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGTGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGATGCGGAGATTGATTACAGCGATATTCCTGCCACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A156JBA1