Detailed information of TA system TA073330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 3135288..3136085
• Replicon: chromosome
• Accession: NZ_CP019839
• Organism: Enterobacter roggenkampii strain R11

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: B1H21_RS15225
• Protein ID: WP_063417750.1
• Coordinates: 3135288..3135809 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: B1H21_RS15230
• Protein ID: WP_008499829.1
• Coordinates: 3135816..3136085 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H21_RS15195	3130871..3131560	+	690	WP_063417746.1	pyrimidine utilization protein B	-
B1H21_RS15200	3131572..3131958	+	387	WP_008499822.1	pyrimidine utilization protein C	-
B1H21_RS15205	3131966..3132766	+	801	WP_063417747.1	pyrimidine utilization protein D	-
B1H21_RS15210	3132776..3133366	+	591	WP_063417748.1	malonic semialdehyde reductase	-
B1H21_RS15215	3133376..3133870	+	495	WP_023292823.1	pyrimidine utilization flavin reductase protein F	-
B1H21_RS15220	3133892..3135214	+	1323	WP_063417749.1	pyrimidine utilization transport protein G	-
B1H21_RS15225	3135288..3135809	-	522	WP_063417750.1	GNAT family N-acetyltransferase	Toxin
B1H21_RS15230	3135816..3136085	-	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
B1H21_RS15235	3136147..3137064	-	918	WP_023292825.1	DMT family transporter	-
B1H21_RS15240	3137183..3137353	-	171	WP_013097201.1	general stress protein	-
B1H21_RS15245	3137745..3138341	+	597	WP_008499831.1	NAD(P)H:quinone oxidoreductase	-
B1H21_RS15250	3138362..3138589	+	228	WP_008499832.1	YccJ family protein	-
B1H21_RS15255	3138625..3139866	-	1242	WP_063417751.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
B1H21_RS15260	3140019..3140462	+	444	WP_008499834.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19656.42 Da
• Isoelectric Point: 7.4313

>T73330 WP_063417750.1 NZ_CP019839:c3135809-3135288 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTGENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T73330 NZ_CP019839:c3135809-3135288 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTAGCATCGCAGGCGGTGGGTGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGGTGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT73330 WP_008499829.1 NZ_CP019839:c3136085-3135816 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT73330 NZ_CP019839:c3136085-3135816 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43