Detailed information of TA system TA073321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 1238063..1238636
• Replicon: chromosome
• Accession: NZ_CP019839
• Organism: Enterobacter roggenkampii strain R11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B1H21_RS06065
• Protein ID: WP_034018908.1
• Coordinates: 1238349..1238636 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: B1H21_RS06060
• Protein ID: WP_084832994.1
• Coordinates: 1238063..1238362 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H21_RS06030	1234110..1234334	-	225	WP_001749832.1	hypothetical protein	-
B1H21_RS06035	1234337..1234555	-	219	WP_029456569.1	hypothetical protein	-
B1H21_RS06040	1234638..1234880	-	243	WP_016943286.1	hypothetical protein	-
B1H21_RS06045	1235225..1235605	+	381	WP_034018902.1	conjugal transfer protein TraJ	-
B1H21_RS06050	1235602..1237554	+	1953	WP_084832992.1	relaxase/mobilization nuclease domain-containing protein	-
B1H21_RS06055	1237711..1237917	-	207	WP_084832993.1	hypothetical protein	-
B1H21_RS06060	1238063..1238362	+	300	WP_084832994.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
B1H21_RS06065	1238349..1238636	+	288	WP_034018908.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1H21_RS06070	1238848..1240050	+	1203	WP_079383775.1	SIR2 family protein	-
B1H21_RS06075	1240037..1241941	+	1905	WP_207554180.1	DUF87 domain-containing protein	-
B1H21_RS24750	1241946..1242164	+	219	WP_165690315.1	KTSC domain-containing protein	-
B1H21_RS06080	1242168..1243199	+	1032	WP_034018910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1227151..1244727	17576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-55)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10872.32 Da
• Isoelectric Point: 4.6533

>T73321 WP_034018908.1 NZ_CP019839:1238349-1238636 [Enterobacter roggenkampii]
VPELEWSQLARADLLAIVDYISDDNPDAAQWLKDDIEAKAAKLPEHPKLYRGGRIEGTREMVVRANYIVVYTEDSFAVRI
LRVLHAAQQWPPTQE

Nucleotide

• Length: 288 bp

>T73321 NZ_CP019839:1238349-1238636 [Enterobacter roggenkampii]
GTGCCCGAGCTTGAATGGTCCCAACTGGCACGAGCGGATTTACTGGCAATTGTCGATTATATCTCAGACGACAATCCTGA
CGCCGCTCAATGGCTGAAAGATGATATCGAAGCCAAGGCAGCTAAGCTTCCGGAACATCCGAAGCTCTATCGAGGGGGGC
GGATAGAGGGCACGCGCGAAATGGTCGTAAGGGCAAACTACATTGTCGTATACACGGAGGACTCTTTTGCCGTGCGTATA
CTGCGTGTGCTGCACGCAGCGCAACAGTGGCCGCCAACTCAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11015.50 Da
• Isoelectric Point: 6.8812

>AT73321 WP_084832994.1 NZ_CP019839:1238063-1238362 [Enterobacter roggenkampii]
MAAQTSMLHIRVDDKLKADATEKLANVGLSVSDAVRIFLTRVTKEGGLPVGLTTDPEAHDKWFRAKVHEALSDSQPDVPH
QQVMDEAQDLIDRKRRARA

Nucleotide

• Length: 300 bp

>AT73321 NZ_CP019839:1238063-1238362 [Enterobacter roggenkampii]
ATGGCTGCTCAAACATCCATGCTGCACATCCGTGTAGACGACAAGCTGAAGGCTGATGCGACCGAAAAGCTCGCTAACGT
TGGTCTATCAGTCTCGGATGCCGTGCGAATTTTCCTGACTCGCGTCACCAAAGAAGGCGGTTTGCCTGTGGGACTGACAA
CTGACCCGGAAGCCCATGACAAGTGGTTCCGCGCCAAGGTGCATGAGGCTTTGTCGGACAGCCAGCCGGATGTACCACAT
CAACAGGTAATGGACGAGGCGCAAGATCTTATCGACAGGAAGCGCCGTGCCCGAGCTTGA