Detailed information of TA system TA073319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 90590..91206
• Replicon: chromosome
• Accession: NZ_CP019839
• Organism: Enterobacter roggenkampii strain R11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B1H21_RS00445
• Protein ID: WP_084832869.1
• Coordinates: 90835..91206 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: B1H21_RS00440
• Protein ID: WP_015569912.1
• Coordinates: 90590..90832 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1H21_RS00410	86009..86479	+	471	WP_045352968.1	hypothetical protein	-
B1H21_RS00415	86568..87167	+	600	WP_063418382.1	glucose-1-phosphatase	-
B1H21_RS00420	87161..88042	+	882	WP_023326335.1	virulence factor BrkB family protein	-
B1H21_RS00425	88039..88476	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B1H21_RS00430	88521..89462	+	942	WP_008501835.1	fatty acid biosynthesis protein FabY	-
B1H21_RS00435	89471..90391	-	921	WP_063418381.1	alpha/beta hydrolase	-
B1H21_RS00440	90590..90832	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
B1H21_RS00445	90835..91206	+	372	WP_084832869.1	type II toxin-antitoxin system VapC family toxin	Toxin
B1H21_RS00450	91246..92175	-	930	WP_008501833.1	formate dehydrogenase accessory protein FdhE	-
B1H21_RS00455	92172..92807	-	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
B1H21_RS00460	92804..93706	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13700.81 Da
• Isoelectric Point: 6.3290

>T73319 WP_084832869.1 NZ_CP019839:90835-91206 [Enterobacter roggenkampii]
MEHMAVFDTNILIDLFNNRVEAADAIENTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T73319 NZ_CP019839:90835-91206 [Enterobacter roggenkampii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GAATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTGATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATCCTAGCTACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT73319 WP_015569912.1 NZ_CP019839:90590-90832 [Enterobacter roggenkampii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT73319 NZ_CP019839:90590-90832 [Enterobacter roggenkampii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGCGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3