Detailed information of TA system TA073311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 34777..35324
• Replicon: plasmid pVbh_BscR1
• Accession: NZ_CP019790
• Organism: Bartonella schoenbuchensis R1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BscR1v2_RS07825
• Protein ID: WP_194284962.1
• Coordinates: 34998..35324 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: E6Z0P2
• Locus tag: BscR1v2_RS07820
• Protein ID: WP_034989862.1
• Coordinates: 34777..34998 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BscR1v2_RS07780 (BscR1v2_016640)	29911..30156	+	246	WP_078690369.1	type II toxin-antitoxin system HicB family antitoxin	-
BscR1v2_RS07785	30267..30602	-	336	WP_078690393.1	hypothetical protein	-
BscR1v2_RS07790 (BscR1v2_016660)	30817..31122	-	306	WP_078690370.1	hypothetical protein	-
BscR1v2_RS07795 (BscR1v2_016670)	31251..32510	-	1260	WP_078690371.1	hypothetical protein	-
BscR1v2_RS07800 (BscR1v2_016680)	32978..33259	+	282	WP_078690372.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BscR1v2_RS07805 (BscR1v2_016690)	33277..33576	+	300	WP_078690373.1	HigA family addiction module antidote protein	-
BscR1v2_RS07810	33671..33873	-	203	Protein_59	tyrosine-type recombinase/integrase	-
BscR1v2_RS07815 (BscR1v2_016700)	33921..34226	-	306	WP_010703129.1	hypothetical protein	-
BscR1v2_RS07820	34777..34998	+	222	WP_034989862.1	antitoxin MazE family protein	Antitoxin
BscR1v2_RS07825 (BscR1v2_016720)	34998..35324	+	327	WP_194284962.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BscR1v2_RS07830 (BscR1v2_016730)	35596..35901	-	306	WP_078690375.1	hypothetical protein	-
BscR1v2_RS07835 (BscR1v2_016740)	36030..37301	-	1272	WP_078690361.1	replication protein C	-
BscR1v2_RS07840 (BscR1v2_016750)	37686..38297	+	612	WP_078690376.1	hypothetical protein	-
BscR1v2_RS07845 (BscR1v2_016760)	38873..39592	-	720	WP_078690394.1	nuclease	-
BscR1v2_RS07850 (BscR1v2_016770)	39695..40009	+	315	WP_010703089.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virB4 / virB11	1..55761	55761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (14-103)

Antitoxin

• Antitoxin MazE-like (7-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11951.25 Da
• Isoelectric Point: 9.2988

>T73311 WP_194284962.1 NZ_CP019790:34998-35324 [Bartonella schoenbuchensis R1]
IMRGSLITVVMQGDFGKPRPALVIQANQFSEHTSITVLPITSTLIAAPLLRITIRPNSENGLLKPSQVMIDKIMTVRCDK
VSSIFGYIHTDEMIQIERCLAVFLGIVK

Nucleotide

• Length: 327 bp

>T73311 NZ_CP019790:34998-35324 [Bartonella schoenbuchensis R1]
ATAATGAGAGGTTCTCTTATAACAGTAGTTATGCAAGGCGATTTTGGCAAACCAAGACCCGCGCTAGTAATCCAAGCTAA
TCAATTTAGTGAGCATACCAGCATCACAGTCTTACCAATTACAAGCACACTTATTGCTGCTCCACTACTGCGCATTACTA
TTCGGCCAAATAGCGAAAATGGTTTGCTAAAACCTTCACAGGTAATGATTGATAAAATTATGACGGTACGATGTGATAAA
GTTAGTTCAATTTTCGGCTATATTCACACAGATGAAATGATACAAATTGAACGCTGTTTAGCTGTATTTTTAGGAATAGT
AAAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8657.97 Da
• Isoelectric Point: 9.4438

>AT73311 WP_034989862.1 NZ_CP019790:34777-34998 [Bartonella schoenbuchensis R1]
MGTMHVNERVQKHRNAQRKAGLRLMQIWVPDIRQPDFAEECRRQCRLVAKTDKDNASMQLFMEQSLKDIDGWT

Nucleotide

• Length: 222 bp

>AT73311 NZ_CP019790:34777-34998 [Bartonella schoenbuchensis R1]
ATGGGTACAATGCATGTTAATGAACGCGTTCAAAAACATCGTAATGCACAAAGAAAAGCTGGATTGCGTTTAATGCAAAT
TTGGGTACCAGATATACGTCAACCGGACTTTGCTGAAGAATGCCGTCGCCAATGTCGCTTAGTAGCAAAGACAGATAAAG
ATAATGCGTCTATGCAACTATTTATGGAGCAATCCCTAAAGGATATTGATGGCTGGACATAA

Structures

Antitoxin

• AlphaFold DB: E6Z0P2