Detailed information of TA system TA073310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 29019..29608
• Replicon: plasmid pVbh_BscR1
• Accession: NZ_CP019790
• Organism: Bartonella schoenbuchensis R1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BscR1v2_RS07770
• Protein ID: WP_078690368.1
• Coordinates: 29276..29608 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: N6UD03
• Locus tag: BscR1v2_RS07765
• Protein ID: WP_010703162.1
• Coordinates: 29019..29276 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BscR1v2_RS07720 (BscR1v2_016500)	24169..24441	-	273	WP_078690365.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
BscR1v2_RS08150 (BscR1v2_016510)	24521..24691	-	171	WP_194284961.1	hypothetical protein	-
BscR1v2_RS08100 (BscR1v2_016520)	24898..25053	-	156	WP_010703154.1	hypothetical protein	-
BscR1v2_RS08105 (BscR1v2_016530)	25053..25271	+	219	WP_141638309.1	hypothetical protein	-
BscR1v2_RS07725 (BscR1v2_016540)	25316..25744	-	429	WP_010703155.1	type II toxin-antitoxin system HicB family antitoxin	-
BscR1v2_RS07730 (BscR1v2_016550)	25808..25999	-	192	WP_010703156.1	hypothetical protein	-
BscR1v2_RS07735 (BscR1v2_016560)	26141..26434	-	294	WP_010703157.1	HigA family addiction module antidote protein	-
BscR1v2_RS07740 (BscR1v2_016570)	26451..26732	-	282	WP_078690366.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BscR1v2_RS07745 (BscR1v2_016580)	27005..27316	-	312	WP_010703159.1	transcriptional regulator	-
BscR1v2_RS07750	27329..27652	-	324	Protein_47	type II toxin-antitoxin system RelE/ParE family toxin	-
BscR1v2_RS07755	27960..28348	+	389	Protein_48	single-stranded DNA-binding protein	-
BscR1v2_RS07760 (BscR1v2_016600)	28419..28946	+	528	WP_078690367.1	hypothetical protein	-
BscR1v2_RS07765 (BscR1v2_016610)	29019..29276	+	258	WP_010703162.1	PemI protein	Antitoxin
BscR1v2_RS07770 (BscR1v2_016620)	29276..29608	+	333	WP_078690368.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BscR1v2_RS07775 (BscR1v2_016630)	29669..29899	-	231	WP_078690392.1	hypothetical protein	-
BscR1v2_RS07780 (BscR1v2_016640)	29911..30156	+	246	WP_078690369.1	type II toxin-antitoxin system HicB family antitoxin	-
BscR1v2_RS07785	30267..30602	-	336	WP_078690393.1	hypothetical protein	-
BscR1v2_RS07790 (BscR1v2_016660)	30817..31122	-	306	WP_078690370.1	hypothetical protein	-
BscR1v2_RS07795 (BscR1v2_016670)	31251..32510	-	1260	WP_078690371.1	hypothetical protein	-
BscR1v2_RS07800 (BscR1v2_016680)	32978..33259	+	282	WP_078690372.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BscR1v2_RS07805 (BscR1v2_016690)	33277..33576	+	300	WP_078690373.1	HigA family addiction module antidote protein	-
BscR1v2_RS07810	33671..33873	-	203	Protein_59	tyrosine-type recombinase/integrase	-
BscR1v2_RS07815 (BscR1v2_016700)	33921..34226	-	306	WP_010703129.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virB4 / virB11	1..55761	55761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11844.98 Da
• Isoelectric Point: 10.6999

>T73310 WP_078690368.1 NZ_CP019790:29276-29608 [Bartonella schoenbuchensis R1]
MKRGEIWLVSLDPSSGYEQKGTRPVLIVSPEAFNRVTKTPVVLPITSGGSFARTAGFAVSLMGMGLHTTGVIRCDQPRAL
DIGARKGKKLETVPVMIMNEVLAKLSTFLT

Nucleotide

• Length: 333 bp

>T73310 NZ_CP019790:29276-29608 [Bartonella schoenbuchensis R1]
ATGAAGCGGGGAGAAATTTGGTTAGTATCGCTTGATCCAAGTTCAGGATATGAGCAAAAAGGAACGCGTCCCGTGCTTAT
TGTATCACCAGAAGCATTTAATCGTGTGACTAAAACACCGGTTGTTTTACCCATTACAAGTGGGGGAAGTTTTGCAAGAA
CAGCGGGTTTTGCTGTTTCATTGATGGGAATGGGATTGCACACAACAGGTGTGATACGCTGTGATCAACCACGTGCTCTC
GATATAGGAGCGCGCAAAGGAAAAAAACTAGAAACGGTTCCTGTCATGATTATGAACGAAGTATTAGCTAAGTTGTCAAC
ATTCCTTACGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9370.81 Da
• Isoelectric Point: 4.0330

>AT73310 WP_010703162.1 NZ_CP019790:29019-29276 [Bartonella schoenbuchensis R1]
MHTTKLRKVGGSVMLSIPPALLDVLHLVENTEVGLTIDNGCLIVQPQIFPSYTLDELLAQCDPLADLSDEETEWLDAKPV
SRELL

Nucleotide

• Length: 258 bp

>AT73310 NZ_CP019790:29019-29276 [Bartonella schoenbuchensis R1]
ATGCACACAACGAAATTGCGTAAAGTTGGAGGGTCAGTTATGCTTTCTATACCACCTGCATTACTTGATGTTTTACACCT
CGTTGAAAATACTGAGGTTGGTTTGACTATTGATAATGGGTGTTTAATTGTACAACCTCAGATATTTCCTAGTTATACTC
TTGACGAATTGTTAGCTCAATGTGATCCTTTGGCTGATCTGAGTGATGAGGAAACAGAATGGCTTGATGCTAAACCTGTT
AGTAGAGAGCTCTTGTAA

Structures

Antitoxin

• AlphaFold DB: N6UD03