Detailed information of TA system TA073305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 702708..703297
• Replicon: chromosome
• Accession: NZ_CP019786
• Organism: Bartonella sp. Raccoon60

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: Bra60_RS03145
• Protein ID: WP_078688582.1
• Coordinates: 702708..703040 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: E6YLN2
• Locus tag: Bra60_RS03150
• Protein ID: WP_035005764.1
• Coordinates: 703040..703297 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra60_RS03100	698893..699357	+	465	WP_078688579.1	50S ribosomal protein L13	-
Bra60_RS03105	699360..699845	+	486	WP_035005774.1	30S ribosomal protein S9	-
Bra60_RS03110	699962..700327	-	366	WP_035005772.1	response regulator	-
Bra60_RS03115	700531..700957	-	427	Protein_616	phage terminase large subunit	-
Bra60_RS03120	700938..701174	-	237	WP_153300880.1	hypothetical protein	-
Bra60_RS03125	701214..701714	+	501	WP_078688580.1	hypothetical protein	-
Bra60_RS03135	701963..702271	+	309	WP_078688581.1	HigA family addiction module antidote protein	-
Bra60_RS03140	702374..702625	-	252	Protein_620	type II toxin-antitoxin system RelE/ParE family toxin	-
Bra60_RS03145	702708..703040	-	333	WP_078688582.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Bra60_RS03150	703040..703297	-	258	WP_035005764.1	antitoxin	Antitoxin
Bra60_RS03155	703400..703843	-	444	WP_194285034.1	hypothetical protein	-
Bra60_RS07350	703798..704049	-	252	WP_194284843.1	HigA family addiction module antidote protein	-
Bra60_RS03160	704065..704346	-	282	WP_078688583.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Bra60_RS03170	704661..704966	-	306	WP_035005762.1	ETC complex I subunit	-
Bra60_RS03180	705695..706384	-	690	WP_078698065.1	hypothetical protein	-
Bra60_RS03185	706381..707538	-	1158	WP_194284806.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11857.92 Da
• Isoelectric Point: 10.5590

>T73305 WP_078688582.1 NZ_CP019786:c703040-702708 [Bartonella sp. Raccoon60]
MKRGEIWFVSLDPSSGYEQKGTRPVLIVSLEAFNRVTKTPIVLPITSGGNFARTAGFAVSLMGAGLHTTGVIRCDQPRAL
DIGARQGKKLETVPPMIMNEVLAKLATFLT

Nucleotide

• Length: 333 bp

>T73305 NZ_CP019786:c703040-702708 [Bartonella sp. Raccoon60]
ATGAAGCGGGGAGAAATTTGGTTTGTATCTCTTGACCCATCTTCAGGCTATGAGCAAAAAGGAACGCGTCCTGTACTAAT
TGTATCACTGGAGGCATTTAATCGTGTAACGAAAACACCCATTGTCTTGCCTATTACAAGCGGAGGAAATTTTGCTAGAA
CAGCAGGTTTTGCTGTATCATTAATGGGAGCGGGATTGCATACAACGGGCGTAATACGTTGTGATCAACCACGTGCTCTT
GATATTGGAGCGCGTCAAGGGAAGAAACTGGAAACAGTTCCACCTATGATTATGAATGAAGTCCTTGCTAAGCTAGCGAC
ATTTCTGACGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9299.61 Da
• Isoelectric Point: 4.1623

>AT73305 WP_035005764.1 NZ_CP019786:c703297-703040 [Bartonella sp. Raccoon60]
MHTTKLRKVGGSVMLSIPPALLDVLHLTENTQVGLAVDNGQLIVKPQTFPSYTLDELLAQCNSSDDFADEDMEWFGAQPV
GKELL

Nucleotide

• Length: 258 bp

>AT73305 NZ_CP019786:c703297-703040 [Bartonella sp. Raccoon60]
ATGCATACAACAAAATTACGTAAAGTTGGAGGATCAGTGATGCTTTCCATACCGCCAGCATTACTTGATGTTCTTCATCT
TACAGAAAATACGCAAGTTGGTTTGGCTGTTGATAATGGACAATTGATTGTGAAACCACAGACATTCCCTAGTTATACTC
TTGATGAATTATTAGCCCAATGTAATTCCTCAGATGATTTTGCAGATGAAGATATGGAGTGGTTTGGTGCTCAACCTGTT
GGTAAAGAGCTGTTGTGA

Structures

Antitoxin

• AlphaFold DB: E6YLN2