Detailed information of TA system TA073296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 648148..648737
• Replicon: chromosome
• Accession: NZ_CP019780
• Organism: Bartonella sp. A1379B isolate French red fox A1379B

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: E6YLN3
• Locus tag: BA1379B_RS02810
• Protein ID: WP_035005765.1
• Coordinates: 648148..648480 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: E6YLN2
• Locus tag: BA1379B_RS02815
• Protein ID: WP_035005764.1
• Coordinates: 648480..648737 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA1379B_RS02765	643931..644395	+	465	WP_035006670.1	50S ribosomal protein L13	-
BA1379B_RS02770	644398..644883	+	486	WP_035005774.1	30S ribosomal protein S9	-
BA1379B_RS02775	645000..645365	-	366	WP_035005772.1	response regulator	-
BA1379B_RS02780	645872..646225	-	354	WP_035005768.1	hypothetical protein	-
BA1379B_RS02785	646487..646819	+	333	WP_035005770.1	hypothetical protein	-
BA1379B_RS02790	646800..647051	+	252	Protein_544	PBSX family phage terminase large subunit	-
BA1379B_RS02800	647472..647712	+	241	Protein_545	addiction module antidote protein, HigA family	-
BA1379B_RS02805	647815..648060	-	246	Protein_546	type II toxin-antitoxin system RelE/ParE family toxin	-
BA1379B_RS02810	648148..648480	-	333	WP_035005765.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BA1379B_RS02815	648480..648737	-	258	WP_035005764.1	antitoxin	Antitoxin
BA1379B_RS06865	649207..649488	-	282	Protein_549	HigA family addiction module antidote protein	-
BA1379B_RS02825	649504..649786	-	283	Protein_550	type II toxin-antitoxin system RelE/ParE family toxin	-
BA1379B_RS02835	650102..650407	-	306	WP_035005762.1	ETC complex I subunit	-
BA1379B_RS02845	651134..651823	-	690	WP_035005761.1	hypothetical protein	-
BA1379B_RS02850	651820..652977	-	1158	WP_172802078.1	AI-2E family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11957.06 Da
• Isoelectric Point: 10.8037

>T73296 WP_035005765.1 NZ_CP019780:c648480-648148 [Bartonella sp. A1379B]
MKRGEIWFVSLDPSSGYEQKGTRPVLIVSLEAFNRVTKTPIVLPITSGRNFARTAGFAVSLMGAGLHTTGVIRCDQPRAL
DIGARQGKKLETVPPMIMNEVLAKLATFLT

Nucleotide

• Length: 333 bp

>T73296 NZ_CP019780:c648480-648148 [Bartonella sp. A1379B]
ATGAAGCGGGGAGAAATTTGGTTTGTATCTCTTGACCCATCTTCAGGCTATGAGCAAAAAGGAACGCGTCCTGTACTAAT
TGTATCACTGGAGGCATTTAATCGTGTAACGAAAACACCTATTGTCTTGCCTATTACAAGCGGAAGAAATTTTGCTAGAA
CAGCAGGTTTTGCTGTATCATTAATGGGAGCGGGATTGCATACAACGGGCGTAATACGTTGTGATCAACCACGTGCTCTT
GATATTGGAGCGCGTCAAGGGAAGAAACTGGAAACAGTTCCACCTATGATTATGAATGAAGTCCTTGCTAAGCTAGCGAC
ATTTCTGACGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9299.61 Da
• Isoelectric Point: 4.1623

>AT73296 WP_035005764.1 NZ_CP019780:c648737-648480 [Bartonella sp. A1379B]
MHTTKLRKVGGSVMLSIPPALLDVLHLTENTQVGLAVDNGQLIVKPQTFPSYTLDELLAQCNSSDDFADEDMEWFGAQPV
GKELL

Nucleotide

• Length: 258 bp

>AT73296 NZ_CP019780:c648737-648480 [Bartonella sp. A1379B]
ATGCATACAACAAAATTACGTAAAGTTGGAGGATCAGTGATGCTTTCCATACCGCCAGCATTACTTGATGTTCTTCATCT
TACAGAAAATACGCAAGTTGGTTTGGCTGTTGATAATGGACAATTGATTGTGAAACCACAGACATTCCCTAGTTATACTC
TTGATGAATTATTAGCCCAATGTAATTCCTCAGATGATTTTGCAGATGAAGATATGGAGTGGTTTGGTGCTCAACCTGTT
GGTAAAGAGCTGTTGTGA

Structures

Toxin

• AlphaFold DB: E6YLN3

Antitoxin

• AlphaFold DB: E6YLN2