Detailed information of TA system TA073294

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5011274..5012106
• Replicon: chromosome
• Accession: NZ_CP019778
• Organism: Escherichia coli strain NCTC86

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: B0915_RS25735
• Protein ID: WP_078046160.1
• Coordinates: 5011732..5012106 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: B0915_RS25730
• Protein ID: WP_001540478.1
• Coordinates: 5011274..5011642 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0915_RS25690	5008392..5008538	+	147	WP_059333225.1	DUF905 family protein	-
B0915_RS25700	5008705..5009523	+	819	WP_078046159.1	DUF945 domain-containing protein	-
B0915_RS26995	5009523..5009696	+	174	WP_001183321.1	hypothetical protein	-
B0915_RS25710	5009862..5010335	+	474	WP_021532903.1	antirestriction protein	-
B0915_RS25715	5010351..5010827	+	477	WP_001186786.1	RadC family protein	-
B0915_RS25720	5010890..5011111	+	222	WP_021532904.1	DUF987 domain-containing protein	-
B0915_RS25730	5011274..5011642	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B0915_RS25735	5011732..5012106	+	375	WP_078046160.1	TA system toxin CbtA family protein	Toxin
B0915_RS25740	5012103..5012594	+	492	WP_001586604.1	hypothetical protein	-
B0915_RS25745	5012611..5012787	+	177	WP_001586605.1	DUF957 domain-containing protein	-
B0915_RS27000	5012887..5013045	+	159	WP_001467148.1	hypothetical protein	-
B0915_RS25750	5013698..5016589	+	2892	WP_000580534.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13782.69 Da
• Isoelectric Point: 7.9047

>T73294 WP_078046160.1 NZ_CP019778:5011732-5012106 [Escherichia coli]
MKTVPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T73294 NZ_CP019778:5011732-5012106 [Escherichia coli]
ATGAAAACAGTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT73294 WP_001540478.1 NZ_CP019778:5011274-5011642 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73294 NZ_CP019778:5011274-5011642 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NQ68