Detailed information of TA system TA073293

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4735612..4736447
• Replicon: chromosome
• Accession: NZ_CP019778
• Organism: Escherichia coli strain NCTC86

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A5F0P695
• Locus tag: B0915_RS24180
• Protein ID: WP_022645116.1
• Coordinates: 4735612..4735989 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B0915_RS24185
• Protein ID: WP_078046143.1
• Coordinates: 4736079..4736447 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0915_RS26740	4731978..4733517	-	1540	Protein_4402	lysine decarboxylation/transport transcriptional activator CadC	-
B0915_RS24170	4734266..4735108	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
B0915_RS27040	4735193..4735387	-	195	WP_024181941.1	DUF957 domain-containing protein	-
B0915_RS27045	4735466..4735615	-	150	Protein_4405	hypothetical protein	-
B0915_RS24180	4735612..4735989	-	378	WP_022645116.1	TA system toxin CbtA family protein	Toxin
B0915_RS24185	4736079..4736447	-	369	WP_078046143.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B0915_RS24195	4736610..4736831	-	222	WP_000692345.1	DUF987 domain-containing protein	-
B0915_RS24200	4736894..4737370	-	477	WP_022645117.1	RadC family protein	-
B0915_RS24205	4737386..4737865	-	480	WP_001564060.1	antirestriction protein	-
B0915_RS24210	4738131..4738949	-	819	WP_001175165.1	DUF945 domain-containing protein	-
B0915_RS24215	4739039..4739272	-	234	WP_001278293.1	DUF905 family protein	-
B0915_RS24220	4739278..4739955	-	678	WP_001097302.1	hypothetical protein	-
B0915_RS24225	4740103..4740783	-	681	WP_001282921.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4724490..4770203	45713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14021.99 Da
• Isoelectric Point: 7.8045

>T73293 WP_022645116.1 NZ_CP019778:c4735989-4735612 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T73293 NZ_CP019778:c4735989-4735612 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGTTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13602.39 Da
• Isoelectric Point: 6.7390

>AT73293 WP_078046143.1 NZ_CP019778:c4736447-4736079 [Escherichia coli]
VSDTLSGATHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73293 NZ_CP019778:c4736447-4736079 [Escherichia coli]
GTGTCAGACACACTTTCCGGGGCAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A5F0P695