Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3299060..3299714 Replicon chromosome
Accession NZ_CP019778
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag B0915_RS16875 Protein ID WP_000244777.1
Coordinates 3299060..3299467 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B0915_RS16880 Protein ID WP_000354046.1
Coordinates 3299448..3299714 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0915_RS16855 3295017..3296750 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
B0915_RS16860 3296756..3297466 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B0915_RS16865 3297491..3298387 - 897 WP_042091714.1 site-specific tyrosine recombinase XerD -
B0915_RS16870 3298499..3299020 + 522 WP_001055874.1 flavodoxin FldB -
B0915_RS16875 3299060..3299467 - 408 WP_000244777.1 toxin CptA Toxin
B0915_RS16880 3299448..3299714 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B0915_RS16885 3299957..3300937 + 981 WP_001514522.1 tRNA-modifying protein YgfZ -
B0915_RS16890 3301133..3301792 - 660 WP_000250274.1 hemolysin III family protein -
B0915_RS16895 3301956..3302267 - 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
B0915_RS16900 3302312..3303745 + 1434 WP_001514525.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T73287 WP_000244777.1 NZ_CP019778:c3299467-3299060 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73287 NZ_CP019778:c3299467-3299060 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73287 WP_000354046.1 NZ_CP019778:c3299714-3299448 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73287 NZ_CP019778:c3299714-3299448 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References