Detailed information of TA system TA073284

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2363834..2364666
• Replicon: chromosome
• Accession: NZ_CP019778
• Organism: Escherichia coli strain NCTC86

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: B0915_RS12360
• Protein ID: WP_000854765.1
• Coordinates: 2364292..2364666 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: B0915_RS12355
• Protein ID: WP_001360327.1
• Coordinates: 2363834..2364202 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0915_RS12310	2359423..2360994	-	1572	WP_000381395.1	IS66 family transposase	-
B0915_RS12315	2361014..2361361	-	348	WP_078046020.1	IS66 family insertion sequence element accessory protein TnpB	-
B0915_RS12320	2361361..2362038	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
B0915_RS12335	2362422..2362895	+	474	WP_000855059.1	antirestriction protein	-
B0915_RS12340	2362911..2363387	+	477	WP_001186774.1	RadC family protein	-
B0915_RS12345	2363450..2363671	+	222	WP_023356553.1	DUF987 domain-containing protein	-
B0915_RS12355	2363834..2364202	+	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B0915_RS12360	2364292..2364666	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
B0915_RS12365	2364663..2364869	+	207	WP_000976829.1	hypothetical protein	-
B0915_RS26575	2364882..2364995	+	114	WP_001161660.1	DUF957 domain-containing protein	-
B0915_RS12370	2365259..2366005	-	747	Protein_2254	IS21-like element ISEc12 family helper ATPase IstB	-
B0915_RS12375	2366020..2367561	-	1542	WP_001445980.1	IS21-like element ISEc12 family transposase	-
B0915_RS27035	2368379..2368768	+	390	WP_077249349.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2359423..2370907	11484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T73284 WP_000854765.1 NZ_CP019778:2364292-2364666 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T73284 NZ_CP019778:2364292-2364666 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT73284 WP_001360327.1 NZ_CP019778:2363834-2364202 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT73284 NZ_CP019778:2363834-2364202 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: A0A7U9IW59