Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1770129..1770767 Replicon chromosome
Accession NZ_CP019778
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag B0915_RS09260 Protein ID WP_000813794.1
Coordinates 1770129..1770305 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag B0915_RS09265 Protein ID WP_001270286.1
Coordinates 1770351..1770767 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0915_RS09240 1765748..1766962 - 1215 WP_078045984.1 BenE family transporter YdcO -
B0915_RS09245 1767015..1767551 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
B0915_RS09250 1767624..1769585 + 1962 WP_066009051.1 23S rRNA 5-hydroxycytidine C2501 synthase -
B0915_RS09255 1769677..1769907 - 231 WP_000494244.1 YncJ family protein -
B0915_RS09260 1770129..1770305 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
B0915_RS09265 1770351..1770767 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
B0915_RS09270 1770846..1772252 + 1407 WP_078045985.1 PLP-dependent aminotransferase family protein -
B0915_RS09275 1772497..1773642 + 1146 WP_078045986.1 ABC transporter substrate-binding protein -
B0915_RS09280 1773660..1774673 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
B0915_RS09285 1774674..1775615 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T73274 WP_000813794.1 NZ_CP019778:1770129-1770305 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T73274 NZ_CP019778:1770129-1770305 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT73274 WP_001270286.1 NZ_CP019778:1770351-1770767 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT73274 NZ_CP019778:1770351-1770767 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References