Detailed information of TA system TA073273

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1318147..1318945
• Replicon: chromosome
• Accession: NZ_CP019778
• Organism: Escherichia coli strain NCTC86

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: B0915_RS06725
• Protein ID: WP_066009386.1
• Coordinates: 1318568..1318945 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7UP42
• Locus tag: B0915_RS06720
• Protein ID: WP_001280955.1
• Coordinates: 1318147..1318521 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0915_RS06675	1313502..1314407	+	906	WP_000203541.1	chemotaxis protein	-
B0915_RS06680	1314404..1315474	+	1071	WP_053886242.1	patatin-like phospholipase family protein	-
B0915_RS06695	1315814..1316632	+	819	WP_001234682.1	DUF945 domain-containing protein	-
B0915_RS06700	1316723..1317208	+	486	WP_023910464.1	antirestriction protein	-
B0915_RS06705	1317224..1317700	+	477	WP_001186192.1	RadC family protein	-
B0915_RS06710	1317763..1317984	+	222	WP_001220314.1	DUF987 domain-containing protein	-
B0915_RS06720	1318147..1318521	+	375	WP_001280955.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B0915_RS06725	1318568..1318945	+	378	WP_066009386.1	TA system toxin CbtA family protein	Toxin
B0915_RS06730	1318942..1319430	+	489	WP_044704957.1	hypothetical protein	-
B0915_RS26425	1319442..1319537	+	96	WP_023910462.1	DUF957 domain-containing protein	-
B0915_RS06735	1319586..1320341	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
B0915_RS06740	1320338..1321837	-	1500	WP_001735705.1	IS21-like element ISEc10 family transposase	-
B0915_RS06745	1321891..1322718	+	828	WP_031326415.1	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	1318147..1331658	13511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14247.32 Da
• Isoelectric Point: 8.2905

>T73273 WP_066009386.1 NZ_CP019778:1318568-1318945 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T73273 NZ_CP019778:1318568-1318945 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13767.59 Da
• Isoelectric Point: 6.6248

>AT73273 WP_001280955.1 NZ_CP019778:1318147-1318521 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT73273 NZ_CP019778:1318147-1318521 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGTGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9P0D0