Detailed information of TA system TA073272

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1107020..1107864
• Replicon: chromosome
• Accession: NZ_CP019778
• Organism: Escherichia coli strain NCTC86

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: B0915_RS05690
• Protein ID: WP_000854686.1
• Coordinates: 1107481..1107864 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: B0915_RS05685
• Protein ID: WP_001285602.1
• Coordinates: 1107020..1107400 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0915_RS05645	1103268..1103723	+	456	WP_000581502.1	hypothetical protein	-
B0915_RS05650	1103802..1104035	+	234	WP_001119727.1	DUF905 family protein	-
B0915_RS05660	1104136..1104954	+	819	WP_068879456.1	DUF945 domain-containing protein	-
B0915_RS05665	1105046..1105531	+	486	WP_000214307.1	antirestriction protein	-
B0915_RS05670	1105547..1106023	+	477	WP_021543704.1	RadC family protein	-
B0915_RS05675	1106086..1106307	+	222	WP_000692298.1	DUF987 domain-containing protein	-
B0915_RS05680	1106326..1107009	+	684	WP_000086768.1	hypothetical protein	-
B0915_RS05685	1107020..1107400	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B0915_RS05690	1107481..1107864	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
B0915_RS05695	1107861..1108349	+	489	WP_001054233.1	hypothetical protein	-
B0915_RS05700	1108366..1108563	+	198	WP_000839256.1	DUF957 domain-containing protein	-
B0915_RS05705	1108648..1109490	+	843	WP_068879455.1	DUF4942 domain-containing protein	-
B0915_RS05715	1109982..1110200	-	219	WP_001040187.1	translation initiation factor IF-1	-
B0915_RS05725	1110485..1111189	-	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T73272 WP_000854686.1 NZ_CP019778:1107481-1107864 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T73272 NZ_CP019778:1107481-1107864 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT73272 WP_001285602.1 NZ_CP019778:1107020-1107400 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT73272 NZ_CP019778:1107020-1107400 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG