Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 494224..494842 Replicon chromosome
Accession NZ_CP019777
Organism Escherichia coli NU14

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag B0908_RS02405 Protein ID WP_001291435.1
Coordinates 494224..494442 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag B0908_RS02410 Protein ID WP_000344800.1
Coordinates 494468..494842 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0908_RS02370 489511..490083 + 573 WP_000779831.1 YbaY family lipoprotein -
B0908_RS02375 490114..490425 - 312 WP_000409908.1 MGMT family protein -
B0908_RS02385 490804..491157 + 354 WP_000878140.1 DUF1428 domain-containing protein -
B0908_RS02390 491199..492749 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
B0908_RS02395 492913..493383 - 471 WP_000136192.1 YlaC family protein -
B0908_RS02400 493499..494050 - 552 WP_000102543.1 maltose O-acetyltransferase -
B0908_RS02405 494224..494442 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
B0908_RS02410 494468..494842 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
B0908_RS02415 495387..498536 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
B0908_RS02420 498559..499752 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T73243 WP_001291435.1 NZ_CP019777:c494442-494224 [Escherichia coli NU14]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T73243 NZ_CP019777:c494442-494224 [Escherichia coli NU14]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT73243 WP_000344800.1 NZ_CP019777:c494842-494468 [Escherichia coli NU14]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT73243 NZ_CP019777:c494842-494468 [Escherichia coli NU14]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References