73237

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 65292..65906 Replicon plasmid unnamed
Accession NZ_CP019733
Organism Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08

Toxin (Protein)


Gene name PfiT Uniprot ID A0A2V0R8Y7
Locus tag B1F85_RS31600 Protein ID WP_017685123.1
Coordinates 65556..65906 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID S6SUK0
Locus tag B1F85_RS31595 Protein ID WP_017685122.1
Coordinates 65292..65540 (+) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1F85_RS31575 61310..61648 - 339 WP_017685230.1 hypothetical protein -
B1F85_RS32825 61785..62072 - 288 WP_153303634.1 hypothetical protein -
B1F85_RS31580 62154..62546 - 393 WP_017685201.1 hypothetical protein -
B1F85_RS31585 62724..63791 + 1068 WP_074556923.1 IS630 family transposase -
B1F85_RS31590 63989..64951 - 963 WP_017685121.1 tyrosine-type recombinase/integrase -
B1F85_RS31595 65292..65540 + 249 WP_017685122.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B1F85_RS31600 65556..65906 + 351 WP_017685123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B1F85_RS31605 66038..66622 - 585 Protein_72 helix-turn-helix transcriptional regulator -
B1F85_RS31615 68329..68634 + 306 WP_017706770.1 type IV secretion system protein -
B1F85_RS31620 68624..69433 + 810 WP_017684942.1 P-type conjugative transfer protein VirB9 -
B1F85_RS31625 69420..70790 + 1371 WP_017684943.1 TrbI/VirB10 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74460 74460
- inside IScluster/Tn - - 52136..68172 16036


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-101)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 13012.89 Da        Isoelectric Point: 4.1953

>T73237 WP_017685123.1 NZ_CP019733:65556-65906 [Pseudomonas syringae pv. actinidiae]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T73237 NZ_CP019733:65556-65906 [Pseudomonas syringae pv. actinidiae]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9389.72 Da        Isoelectric Point: 9.5019

>AT73237 WP_017685122.1 NZ_CP019733:65292-65540 [Pseudomonas syringae pv. actinidiae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Download         Length: 249 bp

>AT73237 NZ_CP019733:65292-65540 [Pseudomonas syringae pv. actinidiae]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2V0R8Y7


Antitoxin

Source ID Structure
AlphaFold DB A0A2V0R8I6

References