Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | PfiT-PfiA/ParE-Phd |
Location | 65292..65906 | Replicon | plasmid unnamed |
Accession | NZ_CP019733 | ||
Organism | Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08 |
Toxin (Protein)
Gene name | PfiT | Uniprot ID | A0A2V0R8Y7 |
Locus tag | B1F85_RS31600 | Protein ID | WP_017685123.1 |
Coordinates | 65556..65906 (+) | Length | 117 a.a. |
Antitoxin (Protein)
Gene name | PfiA | Uniprot ID | S6SUK0 |
Locus tag | B1F85_RS31595 | Protein ID | WP_017685122.1 |
Coordinates | 65292..65540 (+) | Length | 83 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
B1F85_RS31575 | 61310..61648 | - | 339 | WP_017685230.1 | hypothetical protein | - |
B1F85_RS32825 | 61785..62072 | - | 288 | WP_153303634.1 | hypothetical protein | - |
B1F85_RS31580 | 62154..62546 | - | 393 | WP_017685201.1 | hypothetical protein | - |
B1F85_RS31585 | 62724..63791 | + | 1068 | WP_074556923.1 | IS630 family transposase | - |
B1F85_RS31590 | 63989..64951 | - | 963 | WP_017685121.1 | tyrosine-type recombinase/integrase | - |
B1F85_RS31595 | 65292..65540 | + | 249 | WP_017685122.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
B1F85_RS31600 | 65556..65906 | + | 351 | WP_017685123.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
B1F85_RS31605 | 66038..66622 | - | 585 | Protein_72 | helix-turn-helix transcriptional regulator | - |
B1F85_RS31615 | 68329..68634 | + | 306 | WP_017706770.1 | type IV secretion system protein | - |
B1F85_RS31620 | 68624..69433 | + | 810 | WP_017684942.1 | P-type conjugative transfer protein VirB9 | - |
B1F85_RS31625 | 69420..70790 | + | 1371 | WP_017684943.1 | TrbI/VirB10 family protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | - | 1..74460 | 74460 | |
- | inside | IScluster/Tn | - | - | 52136..68172 | 16036 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13012.89 Da Isoelectric Point: 4.1953
>T73237 WP_017685123.1 NZ_CP019733:65556-65906 [Pseudomonas syringae pv. actinidiae]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI
Download Length: 351 bp
>T73237 NZ_CP019733:65556-65906 [Pseudomonas syringae pv. actinidiae]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG
Antitoxin
Download Length: 83 a.a. Molecular weight: 9389.72 Da Isoelectric Point: 9.5019
>AT73237 WP_017685122.1 NZ_CP019733:65292-65540 [Pseudomonas syringae pv. actinidiae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK
Download Length: 249 bp
>AT73237 NZ_CP019733:65292-65540 [Pseudomonas syringae pv. actinidiae]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2V0R8Y7 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2V0R8I6 |