73231

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 640800..642529 Replicon chromosome
Accession NZ_CP019732
Organism Pseudomonas syringae pv. actinidiae strain CRAFRU 14.08

Toxin (Protein)


Gene name pmenT Uniprot ID A0A2V0QPP4
Locus tag B1F85_RS03320 Protein ID WP_003379114.1
Coordinates 641885..642529 (-) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A2V0QNZ0
Locus tag B1F85_RS03315 Protein ID WP_003379115.1
Coordinates 640800..641888 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1F85_RS03295 636436..637278 - 843 WP_003379119.1 glutathione-dependent disulfide-bond oxidoreductase -
B1F85_RS03300 637549..638616 + 1068 WP_032685463.1 IS630 family transposase -
B1F85_RS03305 638783..639559 + 777 WP_017699458.1 ABC transporter permease -
B1F85_RS03310 639556..640260 + 705 WP_017683791.1 ABC transporter ATP-binding protein -
B1F85_RS32860 640341..640594 + 254 Protein_616 glycosyl transferase family 2 -
B1F85_RS03315 640800..641888 - 1089 WP_003379115.1 macro domain-containing protein Antitoxin
B1F85_RS03320 641885..642529 - 645 WP_003379114.1 DUF4433 domain-containing protein Toxin
B1F85_RS03325 642526..643107 - 582 WP_017683790.1 DUF4433 domain-containing protein -
B1F85_RS03330 643525..644646 + 1122 WP_003379112.1 DUF91 domain-containing protein -
B1F85_RS03335 644946..645677 - 732 WP_003379109.1 helix-turn-helix transcriptional regulator -
B1F85_RS03340 646023..646550 - 528 WP_003379107.1 inorganic diphosphatase -
B1F85_RS03345 646661..647470 - 810 WP_017683789.1 zinc-dependent peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 637885..638616 731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24813.38 Da        Isoelectric Point: 6.4920

>T73231 WP_003379114.1 NZ_CP019732:c642529-641885 [Pseudomonas syringae pv. actinidiae]
MIYSSLNPEKALIWRIVHRDNLPWILDNGLHCASSYMQAPQYVNIGNADLIDKRRNRSVPIAPAGVLSDYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLPRIEQLGLAFVFTNAHAYPDWTDYYSDLVNLDQIDWPIIQKRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYDDAMKERIEQDVAARGLTLPVHARPGWYFQ

Download         Length: 645 bp

>T73231 NZ_CP019732:c642529-641885 [Pseudomonas syringae pv. actinidiae]
ATGATCTACAGCAGTCTGAATCCAGAAAAAGCTCTGATTTGGCGAATCGTCCACCGCGATAATCTGCCTTGGATTCTGGA
CAATGGCCTGCATTGCGCAAGCTCCTACATGCAAGCACCGCAGTACGTGAACATCGGCAACGCTGACCTGATCGACAAAC
GCCGCAATCGCAGTGTTCCAATTGCTCCAGCGGGCGTACTGTCCGACTACGTGCCCTTCTACTTCACACCTTTCTCTGTG
ATGATGAAAAATATCCATTCCGGCTGGAGCGTTCAGCAGCGTAGCAATGACGAGATAGTGATTCTGGTCTCAAGCCTGCC
TCGTATTGAGCAGCTTGGCCTGGCGTTCGTCTTTACCAACGCACACGCCTACCCTGACTGGACCGATTACTACAGCGATC
TGGTGAATCTCGACCAGATCGACTGGCCTATAATCCAGAAACGAGACTTCAAGCGTGACCCTGATGATCCGCGTAAGATG
GAGCGTTATCAGGCCGAAGCGCTCATTCACCATCACCTACCGATTACAGGACTCCTGGGCATCATGTGCTACGACGATGC
AATGAAAGAACGCATTGAGCAAGACGTTGCCGCCCGAGGTCTGACTTTGCCTGTCCACGCGCGTCCGGGATGGTATTTCC
AATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40646.66 Da        Isoelectric Point: 6.1829

>AT73231 WP_003379115.1 NZ_CP019732:c641888-640800 [Pseudomonas syringae pv. actinidiae]
MIRFTQGNLLEADTEALVNTVNTVGVMGKGIALMFKERFTENYRLYSAACKAGEVETGKVHVTAVNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLRDLRRFLIDNHVKSVAVPPLGAGNGGLKWVEVREQIVDVLSDLDVDVLVFEPTDQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEQLPSAKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGIDDVIWFDEGRKAFLQTYLKTEAKEYSQALERTAELIDGFESPFGMELLATVDWLLCKSGVSPTVPTVREA
LKHWDGGAGAAARKSRLFDDKAIDIALKRLTTSSLSPEMPPS

Download         Length: 1089 bp

>AT73231 NZ_CP019732:c641888-640800 [Pseudomonas syringae pv. actinidiae]
ATGATCAGATTCACCCAAGGCAATCTGCTGGAAGCAGATACCGAAGCTCTCGTCAACACGGTGAACACTGTAGGCGTAAT
GGGAAAAGGCATTGCGCTGATGTTCAAGGAGCGCTTTACCGAAAACTATCGCCTCTATTCTGCGGCGTGCAAGGCGGGTG
AGGTAGAGACCGGAAAGGTTCACGTAACGGCAGTCAACGAGCTGGACGGCCCGCGCTGGATAGTGAATTTCCCTACCAAG
CGTCACTGGCGTTCCCCCTCGCAGATGGCATGGATCACCGAAGGCTTACGGGATCTGCGCCGTTTTTTAATCGATAACCA
CGTGAAGTCCGTAGCGGTTCCACCTCTCGGGGCGGGTAATGGCGGTTTGAAATGGGTAGAGGTCCGTGAGCAGATTGTTG
ATGTCTTGAGTGATCTGGATGTCGATGTACTTGTCTTTGAACCCACTGATCAATATCTGAATGTCGCGAAGCGTAGCGGT
GTCGAGAAACTGACGCCTGCACGCGCACTCATTGCCGAACTCGTTCGGCGTTACTGGGTTTTGGGCATGGAATGCAGCCT
GCTTGAGATTCAGAAGCTCGCTTGGTTTCTGGAGCGTTCGATAGAGCAATTGCCAAGCGCCAAGAACCCCTTGGATCTCA
AATTTGTAGCCCACAAATATGGGCCATACGCCAATCGCCTTGAGCATCTGCTCGACAATCTCGATGGTAGCTACCTGCAT
TGCGATAAACGCATCAGCGACGCAGGTATTGACGACGTGATCTGGTTTGACGAGGGCCGTAAGGCTTTTCTGCAAACTTA
TCTGAAAACAGAGGCCAAGGAATATTCCCAGGCTCTGGAGCGCACCGCAGAGTTGATTGACGGCTTTGAGTCGCCTTTCG
GCATGGAACTGTTGGCGACGGTTGACTGGTTATTGTGTAAGTCGGGAGTGTCTCCAACGGTTCCGACAGTGCGTGAAGCA
TTGAAGCATTGGGATGGAGGCGCAGGTGCGGCAGCTCGGAAAAGCAGGTTGTTTGATGATAAAGCGATCGACATTGCTCT
AAAGCGATTGACTACCAGTAGTCTCAGCCCAGAGATGCCCCCTTCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0QNZ0

References