Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 65292..65906 Replicon plasmid unnamed
Accession NZ_CP019731
Organism Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29

Toxin (Protein)


Gene name PfiT Uniprot ID A0A2V0R8Y7
Locus tag B1R35_RS31565 Protein ID WP_017685123.1
Coordinates 65556..65906 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID S6SUK0
Locus tag B1R35_RS31560 Protein ID WP_017685122.1
Coordinates 65292..65540 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1R35_RS31540 61310..61648 - 339 WP_017685230.1 hypothetical protein -
B1R35_RS32755 61785..62072 - 288 WP_153303634.1 hypothetical protein -
B1R35_RS31545 62154..62546 - 393 WP_017685201.1 hypothetical protein -
B1R35_RS31550 62724..63791 + 1068 WP_074556923.1 IS630 family transposase -
B1R35_RS31555 63989..64951 - 963 WP_017685121.1 tyrosine-type recombinase/integrase -
B1R35_RS31560 65292..65540 + 249 WP_017685122.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B1R35_RS31565 65556..65906 + 351 WP_017685123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B1R35_RS31570 66038..66622 - 585 Protein_72 helix-turn-helix transcriptional regulator -
B1R35_RS31580 68329..68634 + 306 WP_017706770.1 type IV secretion system protein -
B1R35_RS31585 68624..69433 + 810 WP_017684942.1 P-type conjugative transfer protein VirB9 -
B1R35_RS31590 69420..70790 + 1371 WP_017684943.1 TrbI/VirB10 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..74460 74460
- inside IScluster/Tn - - 52137..68172 16035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13012.89 Da        Isoelectric Point: 4.1953

>T73229 WP_017685123.1 NZ_CP019731:65556-65906 [Pseudomonas syringae pv. actinidiae]
MNTFALRFTDVAQQSLEDQVEHLAVTQGFSSAAQRIDILIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMQSVDIDEIVISLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T73229 NZ_CP019731:65556-65906 [Pseudomonas syringae pv. actinidiae]
ATGAATACATTTGCACTGCGCTTTACTGATGTTGCTCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATATCCTCATCGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGAATTTTTTAC
GAGGTGATGCAGTCAGTTGACATCGATGAGATTGTTATATCGCTTGTATTGGGCGGTAAACAAAGCGTTGAACAGGCATT
GATCCGCTACTGCCTTCTTCAGCCTATTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9389.72 Da        Isoelectric Point: 9.5019

>AT73229 WP_017685122.1 NZ_CP019731:65292-65540 [Pseudomonas syringae pv. actinidiae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYAQGKHCSTDELKARLSRRFD
HK

Download         Length: 249 bp

>AT73229 NZ_CP019731:65292-65540 [Pseudomonas syringae pv. actinidiae]
ATGCGAGTTGAAACAATTAGCTATTTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCGCTGGTCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAGGCCCGATTGAGCAGGAGGTTTGAC
CACAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0R8Y7

Antitoxin

Source ID Structure
AlphaFold DB A0A2V0R8I6

References