Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 6232241..6232760 Replicon chromosome
Accession NZ_CP019730
Organism Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29

Toxin (Protein)


Gene name relE Uniprot ID S6W9N2
Locus tag B1R35_RS29775 Protein ID WP_017684310.1
Coordinates 6232241..6232531 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S6UWW5
Locus tag B1R35_RS29780 Protein ID WP_002551444.1
Coordinates 6232521..6232760 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1R35_RS29740 6228571..6229113 + 543 WP_017684306.1 hypothetical protein -
B1R35_RS29745 6229177..6229460 - 284 Protein_5479 type II toxin-antitoxin system RelE/ParE family toxin -
B1R35_RS29750 6229450..6229692 - 243 WP_017684307.1 hypothetical protein -
B1R35_RS29755 6229873..6230487 - 615 WP_017684308.1 histidine phosphatase family protein -
B1R35_RS29760 6230580..6230921 - 342 WP_003380754.1 hypothetical protein -
B1R35_RS29765 6231278..6232015 + 738 WP_017684309.1 hypothetical protein -
B1R35_RS29775 6232241..6232531 - 291 WP_017684310.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B1R35_RS29780 6232521..6232760 - 240 WP_002551444.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B1R35_RS29785 6232936..6233397 - 462 WP_017684311.1 GNAT family N-acetyltransferase -
B1R35_RS29790 6233484..6234053 - 570 WP_017684312.1 GNAT family N-acetyltransferase -
B1R35_RS29795 6234214..6234708 + 495 WP_005621707.1 hypothetical protein -
B1R35_RS29800 6234981..6235328 + 348 WP_017684314.1 helix-turn-helix domain-containing protein -
B1R35_RS29805 6235414..6236688 - 1275 WP_003380766.1 OprD family porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-81)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-64)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11145.98 Da        Isoelectric Point: 10.0984

>T73228 WP_017684310.1 NZ_CP019730:c6232531-6232241 [Pseudomonas syringae pv. actinidiae]
MTYSLEFDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
RERDEVYRKAADRLGG

Download         Length: 291 bp

>T73228 NZ_CP019730:c6232531-6232241 [Pseudomonas syringae pv. actinidiae]
ATGACTTATAGCCTCGAATTTGATGCGCGAGCTCTGAAGGAATGGCACAAATTAGGCGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCAAATCGCCTGCATGCGTTGCCCGACTGCTACAAAATCA
AGCTGCGCAGCAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCTGTGGACAAG
CGTGAGCGAGATGAGGTTTACCGTAAGGCGGCTGATCGGTTGGGTGGGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8955.34 Da        Isoelectric Point: 4.6017

>AT73228 WP_002551444.1 NZ_CP019730:c6232760-6232521 [Pseudomonas syringae pv. actinidiae]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLAAPQRVKVSLDDL

Download         Length: 240 bp

>AT73228 NZ_CP019730:c6232760-6232521 [Pseudomonas syringae pv. actinidiae]
ATGGCGTCTATTAATGTTCGTATCGACGACGATCTCAAGGCTCGTGCTTATCTCGAGCTTGAAAAGCTTGGCGTCACCCC
TTCGGAACTGCTGCGCCAAACCCTGCAGTACGTGGCAGAGCGCGGCCAGTTGCCGTTCAAGACCGTACTGATGACCGAGG
AAGACGAAGCCTTGCTTGCAACAGTGCGCGAGCGCCTTGCGGCTCCGCAACGGGTCAAGGTTTCACTGGATGACTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K8M1I3

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8M1G9

References