Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5264276..5264817 Replicon chromosome
Accession NZ_CP019730
Organism Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29

Toxin (Protein)


Gene name relE Uniprot ID A0A0K8LVV5
Locus tag B1R35_RS25255 Protein ID WP_017683357.1
Coordinates 5264542..5264817 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0K8LU58
Locus tag B1R35_RS25250 Protein ID WP_003382249.1
Coordinates 5264276..5264542 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1R35_RS25235 5259559..5259822 + 264 WP_017683360.1 hypothetical protein -
B1R35_RS25240 5259950..5261830 - 1881 WP_003382247.1 methyl-accepting chemotaxis protein -
B1R35_RS25245 5262189..5264111 + 1923 WP_017683359.1 methyl-accepting chemotaxis protein -
B1R35_RS25250 5264276..5264542 + 267 WP_003382249.1 ribbon-helix-helix domain-containing protein Antitoxin
B1R35_RS25255 5264542..5264817 + 276 WP_017683357.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B1R35_RS25265 5265389..5266657 + 1269 WP_007244158.1 OprD family porin -
B1R35_RS25270 5266836..5267660 + 825 WP_003380380.1 NAD(P)-dependent oxidoreductase -
B1R35_RS25275 5267720..5268604 + 885 WP_003380378.1 SMP-30/gluconolactonase/LRE family protein -
B1R35_RS25280 5268661..5269700 + 1040 Protein_4654 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(8-45)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10770.41 Da        Isoelectric Point: 7.0123

>T73226 WP_017683357.1 NZ_CP019730:5264542-5264817 [Pseudomonas syringae pv. actinidiae]
MELMWTSKTLSDVTRLYDFLAVANQPAAAQTAQKLTAAPIMLLSNPRIGERLEEFEPRDVRRIQIGRYEMRYEIVDSTIY
LLRLWHTREDR

Download         Length: 276 bp

>T73226 NZ_CP019730:5264542-5264817 [Pseudomonas syringae pv. actinidiae]
ATGGAGCTGATGTGGACAAGCAAAACGCTATCTGACGTCACCCGCCTGTATGATTTTTTAGCGGTAGCAAACCAGCCCGC
TGCCGCCCAGACCGCACAGAAGCTTACAGCGGCGCCGATCATGCTCCTTTCCAACCCGCGGATTGGTGAGCGTCTGGAGG
AGTTTGAGCCCCGGGATGTGCGCAGAATTCAGATAGGCCGCTATGAAATGCGCTACGAAATTGTGGACTCAACAATTTAT
CTGTTGCGGCTGTGGCATACACGCGAGGATCGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9934.24 Da        Isoelectric Point: 5.7066

>AT73226 WP_003382249.1 NZ_CP019730:5264276-5264542 [Pseudomonas syringae pv. actinidiae]
MTTKTRSVTAHIPEQLAEKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSLST
ATPLPVPR

Download         Length: 267 bp

>AT73226 NZ_CP019730:5264276-5264542 [Pseudomonas syringae pv. actinidiae]
ATGACAACCAAGACCCGATCTGTAACAGCTCACATACCCGAACAATTGGCTGAAAAAGTCGACCTGATGGCAGAGCGACT
CGAACGCTCTAAAAACTGGATCGTCAAACAGGCCTTGTCTGCATGGATAGATCAGGAGGAAGAACGTAGTCGCCTCACTC
GCGAAGCATTAGCCGATGTCGATGCTGGCCGTGTGATTGACCATCAGGCTGTTCAGGCCTGGGCTGACAGTTTAAGCACC
GCTACACCTCTGCCGGTTCCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K8LVV5

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LU58

References