Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 741443..742116 Replicon chromosome
Accession NZ_CP019730
Organism Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V0QPW6
Locus tag B1R35_RS03780 Protein ID WP_017684243.1
Coordinates 741443..741862 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag B1R35_RS03785 Protein ID WP_003381346.1
Coordinates 741859..742116 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1R35_RS03755 736453..737487 - 1035 WP_017684240.1 sulfotransferase -
B1R35_RS03760 737505..738425 - 921 WP_020313563.1 TauD/TfdA family dioxygenase -
B1R35_RS03765 738571..739218 - 648 WP_017703697.1 histidine phosphatase family protein -
B1R35_RS03770 739559..740494 - 936 WP_017704392.1 syringolide biosynthetic protein AvrD1 -
B1R35_RS03775 740769..741402 - 634 Protein_698 recombinase family protein -
B1R35_RS03780 741443..741862 - 420 WP_017684243.1 type II toxin-antitoxin system VapC family toxin Toxin
B1R35_RS03785 741859..742116 - 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
B1R35_RS03790 742471..743127 + 657 WP_017684244.1 hypothetical protein -
B1R35_RS03795 743175..743429 + 255 WP_003381350.1 hypothetical protein -
B1R35_RS03805 744246..745208 - 963 WP_003383614.1 type III effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 729365..788196 58831


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15152.46 Da        Isoelectric Point: 4.7010

>T73224 WP_017684243.1 NZ_CP019730:c741862-741443 [Pseudomonas syringae pv. actinidiae]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAIETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Download         Length: 420 bp

>T73224 NZ_CP019730:c741862-741443 [Pseudomonas syringae pv. actinidiae]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATTGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGATCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT73224 WP_003381346.1 NZ_CP019730:c742116-741859 [Pseudomonas syringae pv. actinidiae]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT73224 NZ_CP019730:c742116-741859 [Pseudomonas syringae pv. actinidiae]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V0QPW6

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References