Detailed information of TA system TA073217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 23514..24079
• Replicon: plasmid unnamed1
• Accession: NZ_CP019718
• Organism: Paenibacillus larvae subsp. larvae strain Eric_V

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ERICV_RS24350
• Protein ID: WP_172423969.1
• Coordinates: 23514..23843 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ERICV_RS24355
• Protein ID: WP_172424007.1
• Coordinates: 23840..24079 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERICV_RS24305	19434..19676	+	243	WP_172423962.1	hypothetical protein	-
ERICV_RS24310	19694..20371	+	678	WP_172423963.1	N-acetylmuramoyl-L-alanine amidase	-
ERICV_RS24315	20378..20842	+	465	WP_024094413.1	hypothetical protein	-
ERICV_RS24320	20860..21135	+	276	WP_036658889.1	hypothetical protein	-
ERICV_RS24325	21148..21387	+	240	WP_172423964.1	transposase	-
ERICV_RS24330	21475..21744	-	270	WP_172423965.1	hypothetical protein	-
ERICV_RS24335	21784..22026	-	243	WP_172423966.1	hypothetical protein	-
ERICV_RS24340	22019..23002	-	984	WP_172423967.1	ParM/StbA family protein	-
ERICV_RS24345	23197..23487	-	291	WP_172423968.1	hypothetical protein	-
ERICV_RS24350	23514..23843	-	330	WP_172423969.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERICV_RS24355	23840..24079	-	240	WP_172424007.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ERICV_RS24360	24651..25025	-	375	WP_172423970.1	hypothetical protein	-
ERICV_RS24365	25009..25197	-	189	WP_172423971.1	hypothetical protein	-
ERICV_RS24370	25384..25641	-	258	WP_172423972.1	hypothetical protein	-
ERICV_RS24375	25643..26026	-	384	WP_172423973.1	hypothetical protein	-
ERICV_RS24380	26033..26323	-	291	WP_172423974.1	hypothetical protein	-
ERICV_RS24385	26316..26639	-	324	WP_172423975.1	hypothetical protein	-
ERICV_RS24390	26672..26878	-	207	WP_172423976.1	hypothetical protein	-
ERICV_RS24395	26887..27585	-	699	WP_172423977.1	DNA cytosine methyltransferase	-
ERICV_RS24400	27592..27819	-	228	WP_172423978.1	hypothetical protein	-
ERICV_RS24405	28100..28300	-	201	WP_172423979.1	hypothetical protein	-
ERICV_RS24410	28303..28503	-	201	WP_172423980.1	hypothetical protein	-
ERICV_RS24415	28496..28999	-	504	WP_172423981.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46114	46114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-108)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12491.51 Da
• Isoelectric Point: 8.0834

>T73217 WP_172423969.1 NZ_CP019718:c23843-23514 [Paenibacillus larvae subsp. larvae]
VTVIPDRGDIVYMDFNPQSGHEQAGWRPALVLSPKLFNATTNLAVVCPITSRVKNYLFEVHLPDTMKTKGVILVHQLKSA
DWRSRKLEIRERAPQEIIDEVIEKIHTFL

Nucleotide

• Length: 330 bp

>T73217 NZ_CP019718:c23843-23514 [Paenibacillus larvae subsp. larvae]
GTGACAGTTATCCCAGATCGCGGTGATATTGTTTATATGGACTTTAATCCGCAAAGCGGGCATGAACAAGCTGGATGGCG
ACCAGCTCTTGTGTTATCGCCCAAATTATTTAATGCGACAACGAATCTTGCTGTAGTCTGCCCCATCACTTCACGGGTGA
AAAACTACTTATTTGAAGTTCATCTTCCAGACACTATGAAGACAAAAGGTGTTATACTTGTACACCAATTGAAAAGCGCC
GATTGGAGATCGCGGAAATTAGAAATTCGGGAGAGAGCACCACAAGAAATTATTGACGAAGTTATCGAAAAGATTCATAC
TTTCCTATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8980.29 Da
• Isoelectric Point: 6.2179

>AT73217 WP_172424007.1 NZ_CP019718:c24079-23840 [Paenibacillus larvae subsp. larvae]
MSTLVSKWGNSLALRIPRHFAESLNLHEGTEVELKLSEDCLSVKPKKKRYTLEELCASIKDGNQHDLIDFGKPVGEEIW

Nucleotide

• Length: 240 bp

>AT73217 NZ_CP019718:c24079-23840 [Paenibacillus larvae subsp. larvae]
ATGAGTACGCTTGTGAGCAAATGGGGAAATAGTCTGGCACTTCGTATTCCCAGACATTTTGCTGAAAGCTTGAATCTTCA
TGAAGGTACTGAAGTTGAATTAAAGCTATCAGAAGACTGCTTGAGTGTCAAGCCAAAGAAAAAAAGGTATACCCTTGAGG
AACTTTGTGCTTCTATTAAAGATGGCAATCAGCATGACCTTATTGACTTCGGTAAACCTGTTGGGGAGGAAATTTGGTGA