Detailed information of TA system TA073216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3868939..3869534
• Replicon: chromosome
• Accession: NZ_CP019717
• Organism: Paenibacillus larvae subsp. larvae strain Eric_V

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ERICV_RS20100
• Protein ID: WP_023483433.1
• Coordinates: 3869241..3869534 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W2E434
• Locus tag: ERICV_RS20095
• Protein ID: WP_036658190.1
• Coordinates: 3868939..3869208 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERICV_RS20070	3864370..3865656	+	1287	WP_036658196.1	MFS transporter	-
ERICV_RS20075	3865953..3866402	+	450	WP_172423637.1	transposase	-
ERICV_RS20080	3866374..3866538	+	165	WP_172423638.1	hypothetical protein	-
ERICV_RS20090	3868288..3868620	-	333	WP_023483434.1	hypothetical protein	-
ERICV_RS20095	3868939..3869208	+	270	WP_036658190.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ERICV_RS20100	3869241..3869534	+	294	WP_023483433.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERICV_RS20105	3869786..3870076	+	291	WP_023483432.1	metalloregulator ArsR/SmtB family transcription factor	-
ERICV_RS20110	3870073..3872583	+	2511	WP_023483431.1	FAD-dependent oxidoreductase	-
ERICV_RS20115	3872689..3872985	+	297	WP_036658187.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3844436..4062929	218493
	-	flank	IS/Tn	-	-	3867819..3868100	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-95)

Antitoxin

• Antitoxin PemI-like (11-89)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10996.71 Da
• Isoelectric Point: 6.7162

>T73216 WP_023483433.1 NZ_CP019717:3869241-3869534 [Paenibacillus larvae subsp. larvae]
MDFNPQIGHEQAGRRPGLVVSPTSFNRVTGFIVVCPITRTVRNYPYEVPLPSDLMTEGVVLAHQVKSLDWKARKAEVREK
VPQEIIDDVLDLIHTFL

Nucleotide

• Length: 294 bp

>T73216 NZ_CP019717:3869241-3869534 [Paenibacillus larvae subsp. larvae]
ATGGATTTTAACCCGCAGATTGGACATGAACAAGCCGGAAGAAGACCAGGCCTTGTTGTCTCTCCTACATCATTCAATAG
GGTAACAGGCTTTATAGTTGTATGCCCTATTACAAGAACCGTTAGGAATTATCCATATGAAGTTCCCCTCCCTTCCGATC
TTATGACCGAAGGTGTTGTTTTGGCTCATCAAGTAAAAAGCTTGGATTGGAAGGCAAGAAAGGCAGAAGTTCGGGAAAAA
GTACCACAAGAAATTATTGACGATGTACTGGATTTAATTCACACTTTCCTATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10006.46 Da
• Isoelectric Point: 5.7379

>AT73216 WP_036658190.1 NZ_CP019717:3868939-3869208 [Paenibacillus larvae subsp. larvae]
MGKDRERMSVMSTIVSKWGNSLAIRIPSNLAESLNIKEGSEVELSIMDSTLTIKPKKRKPTLEELLAKITDGNHHEEIDF
GKPEGNEVW

Nucleotide

• Length: 270 bp

>AT73216 NZ_CP019717:3868939-3869208 [Paenibacillus larvae subsp. larvae]
ATGGGTAAAGACAGGGAAAGGATGAGTGTAATGAGCACAATTGTGAGCAAATGGGGAAATAGTCTCGCAATCCGCATTCC
AAGTAACCTGGCAGAAAGCCTAAACATAAAAGAGGGTTCTGAAGTTGAATTAAGCATCATGGATAGTACACTAACTATAA
AACCTAAAAAACGTAAGCCAACTCTTGAAGAATTACTGGCAAAAATAACAGACGGTAACCATCATGAGGAAATTGACTTT
GGAAAACCGGAGGGGAACGAGGTTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1TQJ8