Detailed information of TA system TA073215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 459939..460565
• Replicon: chromosome
• Accession: NZ_CP019717
• Organism: Paenibacillus larvae subsp. larvae strain Eric_V

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V9W2Z1
• Locus tag: ERICV_RS02295
• Protein ID: WP_023485406.1
• Coordinates: 460215..460565 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ERICV_RS02290
• Protein ID: WP_172423825.1
• Coordinates: 459939..460211 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERICV_RS02275	456332..456514	+	183	WP_024093121.1	hypothetical protein	-
ERICV_RS02280	457084..458340	+	1257	WP_024093122.1	outer membrane lipoprotein carrier protein LolA	-
ERICV_RS02285	458508..459695	+	1188	WP_023485408.1	alanine racemase	-
ERICV_RS02290	459939..460211	+	273	WP_172423825.1	ribbon-helix-helix protein, CopG family	Antitoxin
ERICV_RS02295	460215..460565	+	351	WP_023485406.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERICV_RS02300	460737..462941	+	2205	WP_172423826.1	helix-hairpin-helix domain-containing protein	-
ERICV_RS02305	463143..463709	-	567	WP_077997616.1	sigma-70 family RNA polymerase sigma factor	-
ERICV_RS02310	464107..464565	+	459	WP_023485404.1	hypothetical protein	-
ERICV_RS02315	465157..465384	+	228	WP_024093128.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12877.80 Da
• Isoelectric Point: 5.1393

>T73215 WP_023485406.1 NZ_CP019717:460215-460565 [Paenibacillus larvae subsp. larvae]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAETHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEDTMRKVNDALQVSVGLIEF

Nucleotide

• Length: 351 bp

>T73215 NZ_CP019717:460215-460565 [Paenibacillus larvae subsp. larvae]
TTGATTGTCAAACGTGGTGATGTTTTCTTTGCGGATCTTTCTCCTGTTGTCGGTTCCGAGCAGGGGGGAGTGAGGCCTGT
TCTTATCATTCAGAACGATATCGGGAACCGGTTTAGCCCGACGGTAATCGTGGCGGCGATTACCGCCCAAATTCAAAAAG
CCAAGCTGCCCACTCATGTGGAGATCGATGCGGAAACACACGGATTTGACCGCGATTCGGTTATCTTGCTGGAACAGATC
CGGACCATTGACAAGCAGCGGTTGACCGATAAAATTACCCATTTAGATGAAGATACCATGCGTAAAGTGAACGATGCTTT
ACAGGTAAGTGTAGGGTTGATAGAATTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10556.15 Da
• Isoelectric Point: 7.2048

>AT73215 WP_172423825.1 NZ_CP019717:459939-460211 [Paenibacillus larvae subsp. larvae]
MHNTKRIMISLPDHLLKEVDGIVEKENSNRSEFIRQAMRLYLLERKKRTLRESMQRGYMEMAKINLNMASEAFQAEEEAD
NTVDRLVSGV

Nucleotide

• Length: 273 bp

>AT73215 NZ_CP019717:459939-460211 [Paenibacillus larvae subsp. larvae]
ATGCATAATACGAAGCGTATCATGATTAGTCTTCCAGACCACCTTCTGAAGGAGGTGGACGGCATCGTCGAGAAAGAAAA
CTCGAATCGCAGCGAGTTCATTCGACAAGCCATGAGATTGTACCTGCTCGAAAGAAAGAAGAGGACCCTCCGTGAATCCA
TGCAGAGAGGCTATATGGAAATGGCGAAAATTAATTTAAATATGGCGTCCGAAGCTTTTCAAGCGGAGGAAGAAGCCGAC
AATACGGTGGACCGCTTAGTGAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A2L1TT28