Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 307923..308529 Replicon chromosome
Accession NZ_CP019699
Organism Novibacillus thermophilus strain SG-1

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag B0W44_RS01695 Protein ID WP_077721203.1
Coordinates 308179..308529 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag B0W44_RS01690 Protein ID WP_077721204.1
Coordinates 307923..308174 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W44_RS01670 303233..303601 + 369 WP_077718502.1 holo-ACP synthase -
B0W44_RS01675 303659..305242 + 1584 WP_077718503.1 NAD(P)H-hydrate dehydratase -
B0W44_RS01680 305268..306299 + 1032 WP_077718504.1 outer membrane lipoprotein carrier protein LolA -
B0W44_RS01685 306572..307720 + 1149 WP_077718505.1 alanine racemase -
B0W44_RS01690 307923..308174 + 252 WP_077721204.1 ribbon-helix-helix protein, CopG family Antitoxin
B0W44_RS01695 308179..308529 + 351 WP_077721203.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B0W44_RS01700 308611..310755 + 2145 WP_077718506.1 RNA-binding transcriptional accessory protein -
B0W44_RS01705 310817..311290 + 474 WP_077718507.1 SprT family protein -
B0W44_RS01740 312142..312606 + 465 WP_077718508.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
B0W44_RS01745 312603..313304 + 702 WP_077718509.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 288069..308529 20460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-35)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12951.94 Da        Isoelectric Point: 4.5626

>T73197 WP_077721203.1 NZ_CP019699:308179-308529 [Novibacillus thermophilus]
MMVKRGDVYFADLSPVIGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKLHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEDIMEEVDEALQISLGLIDF

Download         Length: 351 bp

>T73197 NZ_CP019699:308179-308529 [Novibacillus thermophilus]
TTGATGGTGAAGCGCGGCGACGTTTACTTTGCGGATTTGTCACCGGTCATCGGTTCTGAACAAGGTGGAATCCGCCCCGT
ACTCATTATTCAAAACGATATCGGCAATCGGTTCAGCCCTACTGTCATCGTTGCCGCGATAACGGCACAAATTCAAAAGG
CCAAGCTTCCGACGCACGTGGAGATCGACGCGAAACTGCACGGGTTTGACCGTGATTCGGTTGTCCTGCTGGAGCAGATT
CGCACGATTGACAAACAAAGGTTGACCGACAAAATCACTCACTTAGATGAAGATATCATGGAGGAAGTCGATGAAGCCTT
GCAAATCAGTCTGGGCCTCATCGATTTTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9614.03 Da        Isoelectric Point: 6.2248

>AT73197 WP_077721204.1 NZ_CP019699:307923-308174 [Novibacillus thermophilus]
MISLPQHLLHEVDGMVEAEQSSRSDFIRRAMKQYLQELKKRHIRETMQRGYVEMAKINLNIAAEAFQAEEEADLTLNRLV
SGV

Download         Length: 252 bp

>AT73197 NZ_CP019699:307923-308174 [Novibacillus thermophilus]
ATGATCAGTTTGCCGCAGCACCTTCTTCACGAAGTCGACGGAATGGTAGAAGCGGAGCAGTCCAGCCGAAGTGATTTTAT
ACGTCGTGCGATGAAGCAGTATTTACAAGAGTTGAAAAAGCGCCACATTCGCGAAACGATGCAGCGCGGATACGTGGAAA
TGGCTAAAATTAACCTGAATATCGCTGCGGAAGCGTTTCAAGCGGAGGAAGAAGCGGACCTTACCCTAAACCGTCTGGTT
AGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References