73195

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 100518..101143 Replicon plasmid pInv_75_02_1
Accession NZ_CP019696
Organism Shigella sonnei strain 75/02

Toxin (Protein)


Gene name vapC Uniprot ID Q6XVU6
Locus tag BZ172_RS28335 Protein ID WP_000911318.1
Coordinates 100745..101143 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q31SG2
Locus tag BZ172_RS28330 Protein ID WP_000450530.1
Coordinates 100518..100745 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZ172_RS28325 (BZ172_30255) 96165..100436 - 4272 Protein_127 conjugative transfer relaxase/helicase TraI -
BZ172_RS28330 (BZ172_30260) 100518..100745 + 228 WP_000450530.1 toxin-antitoxin system antitoxin VapB Antitoxin
BZ172_RS28335 (BZ172_30265) 100745..101143 + 399 WP_000911318.1 type II toxin-antitoxin system VapC family toxin Toxin
BZ172_RS28340 (BZ172_30270) 101152..103353 - 2202 WP_000492720.1 type IV conjugative transfer system coupling protein TraD -
BZ172_RS28345 103379..103510 - 132 Protein_131 IS1 family transposase -
BZ172_RS30245 (BZ172_30290) 104775..105113 - 339 Protein_133 antirestriction protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ipaH7.8 / ospD3/senA / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / tviC / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ipaH4.5 1..214565 214565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14845.09 Da        Isoelectric Point: 8.5264

>T73195 WP_000911318.1 NZ_CP019696:100745-101143 [Shigella sonnei]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Download         Length: 399 bp

>T73195 NZ_CP019696:100745-101143 [Shigella sonnei]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8535.59 Da        Isoelectric Point: 4.7513

>AT73195 WP_000450530.1 NZ_CP019696:100518-100745 [Shigella sonnei]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT73195 NZ_CP019696:100518-100745 [Shigella sonnei]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6SD6

Antitoxin

Source ID Structure
AlphaFold DB Q31SG2

References