Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 86661..87196 Replicon plasmid pInv_75_02_1
Accession NZ_CP019696
Organism Shigella sonnei strain 75/02

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag BZ172_RS28235 Protein ID WP_000222766.1
Coordinates 86661..86948 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0I0JNN8
Locus tag BZ172_RS28240 Protein ID WP_001132903.1
Coordinates 86945..87196 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZ172_RS28205 (BZ172_30120) 81837..81934 + 98 Protein_105 IS1 family transposase -
BZ172_RS28210 (BZ172_30125) 82091..82310 + 220 Protein_106 hypothetical protein -
BZ172_RS28215 (BZ172_30130) 82700..84427 + 1728 WP_000597732.1 T3SS effector E3 ubiquitin-protein ligase IpaH1.4 -
BZ172_RS29545 (BZ172_30135) 84457..84612 - 156 Protein_108 IS256 family transposase -
BZ172_RS28220 (BZ172_30140) 84751..84909 - 159 Protein_109 transposase -
BZ172_RS28225 (BZ172_30145) 85031..85219 + 189 WP_000957853.1 hypothetical protein -
BZ172_RS28230 (BZ172_30150) 85229..86428 + 1200 WP_000947184.1 IS91 family transposase -
BZ172_RS28235 (BZ172_30155) 86661..86948 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BZ172_RS28240 (BZ172_30160) 86945..87196 - 252 WP_001132903.1 plasmid stabilization protein Antitoxin
BZ172_RS28250 (BZ172_30170) 88161..89018 - 858 WP_000130974.1 incFII family plasmid replication initiator RepA -
BZ172_RS28255 (BZ172_30175) 89011..89085 - 75 WP_001365705.1 RepA leader peptide Tap -
BZ172_RS28265 (BZ172_30185) 89309..89569 - 261 WP_000083819.1 replication regulatory protein RepA -
BZ172_RS28270 (BZ172_30190) 89809..90162 - 354 Protein_117 DUF2726 domain-containing protein -
BZ172_RS28280 (BZ172_30205) 90219..90916 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
BZ172_RS28285 (BZ172_30210) 90931..91176 - 246 Protein_119 hypothetical protein -
BZ172_RS28290 (BZ172_30215) 91215..91424 - 210 WP_001299729.1 hemolysin expression modulator Hha -
BZ172_RS28295 (BZ172_30220) 91470..91931 - 462 WP_001233865.1 thermonuclease family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ipaH7.8 / ospD3/senA / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / tviC / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaA / ipaJ / ospC3 / ospC2 / ipaH4.5 1..214565 214565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T73194 WP_000222766.1 NZ_CP019696:c86948-86661 [Shigella sonnei]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T73194 NZ_CP019696:c86948-86661 [Shigella sonnei]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9225.44 Da        Isoelectric Point: 4.0927

>AT73194 WP_001132903.1 NZ_CP019696:c87196-86945 [Shigella sonnei]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT73194 NZ_CP019696:c87196-86945 [Shigella sonnei]
ATGCCTAACATCATTCTGTCTGAGACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0I0JNN8

References