Detailed information of TA system TA073182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3136268..3137105
• Replicon: chromosome
• Accession: NZ_CP019689
• Organism: Shigella sonnei strain 75/02

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: BZ172_RS16865
• Protein ID: WP_000227784.1
• Coordinates: 3136268..3136810 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: BZ172_RS16870
• Protein ID: WP_001297137.1
• Coordinates: 3136794..3137105 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ172_RS16825 (3131332)	3131332..3131823	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
BZ172_RS16830 (3131951)	3131951..3133315	-	1365	WP_001000971.1	MFS transporter	-
BZ172_RS16840 (3133434)	3133434..3134131	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
BZ172_RS16845 (3134500)	3134500..3134898	+	399	Protein_3022	SEL1-like repeat protein	-
BZ172_RS16860 (3135646)	3135646..3136212	+	567	Protein_3024	SEL1-like repeat protein	-
BZ172_RS16865 (3136268)	3136268..3136810	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
BZ172_RS16870 (3136794)	3136794..3137105	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
BZ172_RS16875 (3137290)	3137290..3138180	-	891	WP_000971327.1	heme o synthase	-
BZ172_RS16880 (3138192)	3138192..3138521	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BZ172_RS16885 (3138521)	3138521..3139135	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BZ172_RS16890 (3139125)	3139125..3141116	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BZ172_RS16895 (3141138)	3141138..3142085	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T73182 WP_000227784.1 NZ_CP019689:c3136810-3136268 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T73182 NZ_CP019689:c3136810-3136268 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT73182 WP_001297137.1 NZ_CP019689:c3137105-3136794 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT73182 NZ_CP019689:c3137105-3136794 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3