Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2449631..2450128 Replicon chromosome
Accession NZ_CP019689
Organism Shigella sonnei strain 75/02

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag BZ172_RS13255 Protein ID WP_001173343.1
Coordinates 2449955..2450128 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag BZ172_RS13250 Protein ID WP_000398619.1
Coordinates 2449631..2449927 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZ172_RS13225 (2446362) 2446362..2447081 - 720 WP_000611285.1 two-component system response regulator DcuR -
BZ172_RS13230 (2447078) 2447078..2448709 - 1632 WP_001216495.1 sensor histidine kinase -
BZ172_RS13235 (2448890) 2448890..2449120 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
BZ172_RS13240 (2449132) 2449132..2449404 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
BZ172_RS13250 (2449631) 2449631..2449927 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BZ172_RS13255 (2449955) 2449955..2450128 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
BZ172_RS13260 (2450247) 2450247..2451764 - 1518 WP_001295086.1 lysine--tRNA ligase -
BZ172_RS13265 (2452001) 2452001..2453458 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
BZ172_RS13270 (2453517) 2453517..2453774 - 258 Protein_2366 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T73180 WP_001173343.1 NZ_CP019689:2449955-2450128 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T73180 NZ_CP019689:2449955-2450128 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT73180 WP_000398619.1 NZ_CP019689:2449631-2449927 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT73180 NZ_CP019689:2449631-2449927 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References