Detailed information of TA system TA073169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 260785..261380
• Replicon: chromosome
• Accession: NZ_CP019687
• Organism: Paenibacillus larvae subsp. larvae strain ATCC-9545

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BXP28_RS01400
• Protein ID: WP_023483433.1
• Coordinates: 260785..261078 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W2E434
• Locus tag: BXP28_RS01405
• Protein ID: WP_036658190.1
• Coordinates: 261111..261380 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXP28_RS01385	257335..257592	-	258	WP_077584850.1	hypothetical protein	-
BXP28_RS01390	257736..260246	-	2511	WP_023483431.1	FAD-dependent oxidoreductase	-
BXP28_RS01395	260243..260533	-	291	WP_023483432.1	metalloregulator ArsR/SmtB family transcription factor	-
BXP28_RS01400	260785..261078	-	294	WP_023483433.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BXP28_RS01405	261111..261380	-	270	WP_036658190.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BXP28_RS01410	261699..262031	+	333	WP_023483434.1	hypothetical protein	-
BXP28_RS01415	262219..263337	+	1119	Protein_275	IS3 family transposase	-
BXP28_RS01420	263919..264368	-	450	WP_152532860.1	transposase	-
BXP28_RS01425	264602..265888	-	1287	WP_036658196.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	262219..262500	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-95)

Antitoxin

• Antitoxin PemI-like (11-89)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10996.71 Da
• Isoelectric Point: 6.7162

>T73169 WP_023483433.1 NZ_CP019687:c261078-260785 [Paenibacillus larvae subsp. larvae]
MDFNPQIGHEQAGRRPGLVVSPTSFNRVTGFIVVCPITRTVRNYPYEVPLPSDLMTEGVVLAHQVKSLDWKARKAEVREK
VPQEIIDDVLDLIHTFL

Nucleotide

• Length: 294 bp

>T73169 NZ_CP019687:c261078-260785 [Paenibacillus larvae subsp. larvae]
ATGGATTTTAACCCGCAGATTGGACATGAACAAGCCGGAAGAAGACCAGGCCTTGTTGTCTCTCCTACATCATTCAATAG
GGTAACAGGCTTTATAGTTGTATGCCCTATTACAAGAACCGTTAGGAATTATCCATATGAAGTTCCCCTCCCTTCCGATC
TTATGACCGAAGGTGTTGTTTTGGCTCATCAAGTAAAAAGCTTGGATTGGAAGGCAAGAAAGGCAGAAGTTCGGGAAAAA
GTACCACAAGAAATTATTGACGATGTACTGGATTTAATTCACACTTTCCTATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10006.46 Da
• Isoelectric Point: 5.7379

>AT73169 WP_036658190.1 NZ_CP019687:c261380-261111 [Paenibacillus larvae subsp. larvae]
MGKDRERMSVMSTIVSKWGNSLAIRIPSNLAESLNIKEGSEVELSIMDSTLTIKPKKRKPTLEELLAKITDGNHHEEIDF
GKPEGNEVW

Nucleotide

• Length: 270 bp

>AT73169 NZ_CP019687:c261380-261111 [Paenibacillus larvae subsp. larvae]
ATGGGTAAAGACAGGGAAAGGATGAGTGTAATGAGCACAATTGTGAGCAAATGGGGAAATAGTCTCGCAATCCGCATTCC
AAGTAACCTGGCAGAAAGCCTAAACATAAAAGAGGGTTCTGAAGTTGAATTAAGCATCATGGATAGTACACTAACTATAA
AACCTAAAAAACGTAAGCCAACTCTTGAAGAATTACTGGCAAAAATAACAGACGGTAACCATCATGAGGAAATTGACTTT
GGAAAACCGGAGGGGAACGAGGTTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1TQJ8