Detailed information of TA system TA073158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1841936..1842495
• Replicon: chromosome
• Accession: NZ_CP019679
• Organism: Brucella melitensis strain C-573

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: ADS42_RS09585
• Protein ID: WP_002966807.1
• Coordinates: 1842250..1842495 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: ADS42_RS09580
• Protein ID: WP_004683740.1
• Coordinates: 1841936..1842250 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ADS42_RS09530	1837641..1837844	-	204	WP_004683737.1	hypothetical protein	-
ADS42_RS09535	1837847..1838062	-	216	WP_005968947.1	hypothetical protein	-
ADS42_RS09540	1838059..1838262	-	204	WP_002964088.1	hypothetical protein	-
ADS42_RS09545	1838312..1839016	+	705	WP_005968944.1	hypothetical protein	-
ADS42_RS09550	1839013..1839243	+	231	WP_002964091.1	hypothetical protein	-
ADS42_RS09555	1839240..1839515	+	276	WP_002964092.1	hypothetical protein	-
ADS42_RS09560	1839538..1840014	+	477	WP_002966806.1	hypothetical protein	-
ADS42_RS09565	1840360..1841070	+	711	WP_004683739.1	porin family protein	-
ADS42_RS09570	1841418..1841588	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
ADS42_RS09580	1841936..1842250	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
ADS42_RS09585	1842250..1842495	-	246	WP_002966807.1	BrnT family toxin	Toxin
ADS42_RS09590	1842628..1843266	+	639	Protein_1757	tRNA-guanine transglycosylase	-
ADS42_RS17090	1843336..1844094	+	759	Protein_1758	IS5 family transposase	-
ADS42_RS09605	1844095..1844787	+	693	WP_006135935.1	hypothetical protein	-
ADS42_RS09610	1845199..1846650	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	1835869..1849966	14097
	-	flank	IS/Tn	-	-	1843336..1843704	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T73158 WP_002966807.1 NZ_CP019679:c1842495-1842250 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T73158 NZ_CP019679:c1842495-1842250 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT73158 WP_004683740.1 NZ_CP019679:c1842250-1841936 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT73158 NZ_CP019679:c1842250-1841936 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1