Detailed information of TA system TA073154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1156345..1156991
• Replicon: chromosome
• Accession: NZ_CP019672
• Organism: Burkholderia cenocepacia strain VC12308

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8E75_RS05420
• Protein ID: WP_077190716.1
• Coordinates: 1156584..1156991 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4EGU8
• Locus tag: A8E75_RS05415
• Protein ID: WP_006491146.1
• Coordinates: 1156345..1156587 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E75_RS05400	1152339..1153745	+	1407	WP_006491136.1	aspartate ammonia-lyase	-
A8E75_RS05405	1153823..1155328	+	1506	WP_006491141.1	amino acid permease	-
A8E75_RS05410	1155403..1156086	-	684	WP_077190717.1	phospholipase	-
A8E75_RS05415	1156345..1156587	+	243	WP_006491146.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A8E75_RS05420	1156584..1156991	+	408	WP_077190716.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8E75_RS05425	1157108..1157335	-	228	WP_006491154.1	hypothetical protein	-
A8E75_RS05430	1157393..1159768	+	2376	WP_077190715.1	MCP four helix bundle domain-containing protein	-
A8E75_RS05435	1159791..1160519	+	729	WP_077190835.1	chemotaxis protein	-
A8E75_RS05440	1160516..1161097	+	582	WP_058902967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14858.02 Da
• Isoelectric Point: 6.4900

>T73154 WP_077190716.1 NZ_CP019672:1156584-1156991 [Burkholderia cenocepacia]
MKFLLDTNAVIAILKGEPAILARLHAWHPADFGIPAIVAHALYYGAYKSQRAAANVARVEALQFEVVSLDTEDAQHAGEI
RAHLIAAGTPIGPYDALIAGQARARQLVLVTHNVREFERVPRLQFEDWLAEPRAD

Nucleotide

• Length: 408 bp

>T73154 NZ_CP019672:1156584-1156991 [Burkholderia cenocepacia]
ATGAAGTTCCTGCTGGATACCAACGCGGTGATCGCCATTCTCAAAGGCGAGCCGGCGATACTGGCCCGTTTGCATGCCTG
GCACCCGGCCGATTTCGGGATTCCCGCGATCGTCGCGCACGCGCTTTACTACGGCGCGTACAAGAGCCAGCGAGCGGCGG
CGAACGTCGCGCGTGTCGAGGCGTTGCAGTTCGAGGTCGTATCGCTCGACACCGAGGATGCACAGCATGCGGGTGAAATT
CGCGCGCACCTGATCGCGGCCGGCACGCCGATTGGTCCATACGACGCGTTGATCGCAGGGCAGGCGCGCGCGCGGCAACT
GGTGCTGGTCACGCACAACGTCCGCGAATTCGAGCGCGTTCCGCGGCTGCAATTCGAAGACTGGCTAGCCGAACCGAGGG
CAGACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.02 Da
• Isoelectric Point: 5.0197

>AT73154 WP_006491146.1 NZ_CP019672:1156345-1156587 [Burkholderia cenocepacia]
MDTAKLFKHGGSQAVRLPKDFRFDTAEVRIRRHGAAVILEPMPQDWAWLAPLIGPVDADFEAAATTQPSAQERSGLDVFE

Nucleotide

• Length: 243 bp

>AT73154 NZ_CP019672:1156345-1156587 [Burkholderia cenocepacia]
ATGGATACTGCAAAGCTCTTCAAGCATGGCGGCTCGCAGGCTGTCCGGCTACCGAAGGATTTCCGCTTCGATACGGCCGA
GGTTCGCATCCGCCGCCACGGGGCGGCCGTCATTCTCGAGCCGATGCCGCAAGACTGGGCTTGGCTGGCACCGCTGATCG
GTCCGGTGGACGCCGATTTCGAGGCTGCAGCGACCACACAGCCGTCCGCTCAGGAGCGCTCGGGCCTGGACGTGTTCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: B4EGU8