Detailed information of TA system TA073150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 4506120..4506761
• Replicon: chromosome
• Accession: NZ_CP019670
• Organism: Burkholderia cenocepacia strain VC12802

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B1K4X0
• Locus tag: A8E96_RS25855
• Protein ID: WP_006479524.1
• Coordinates: 4506120..4506458 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A8E96_RS25860
• Protein ID: WP_006490972.1
• Coordinates: 4506462..4506761 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E96_RS25840	4502639..4504072	-	1434	WP_077219915.1	aldehyde dehydrogenase family protein	-
A8E96_RS25845	4504101..4505741	-	1641	WP_011548230.1	acetolactate synthase large subunit	-
A8E96_RS25855	4506120..4506458	+	339	WP_006479524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8E96_RS25860	4506462..4506761	+	300	WP_006490972.1	putative addiction module antidote protein	Antitoxin
A8E96_RS25865	4506810..4508096	-	1287	WP_077219914.1	DUF445 domain-containing protein	-
A8E96_RS25870	4508249..4509469	-	1221	WP_077219913.1	FAD-dependent oxidoreductase	-
A8E96_RS25875	4509459..4509785	-	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
A8E96_RS25880	4509821..4510297	-	477	WP_006479528.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
A8E96_RS25885	4510312..4511583	-	1272	WP_077219912.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12604.47 Da
• Isoelectric Point: 8.9594

>T73150 WP_006479524.1 NZ_CP019670:4506120-4506458 [Burkholderia cenocepacia]
MVDILQSVPYSAPTFSIRTTDVFEAWFAGLPDRVARRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T73150 NZ_CP019670:4506120-4506458 [Burkholderia cenocepacia]
ATGGTTGACATCCTGCAATCCGTGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGAAGCGTGGTT
CGCCGGCCTGCCGGATCGCGTCGCGCGGCGGCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATTACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTCTGCGGCGGCGATAAGTCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10608.04 Da
• Isoelectric Point: 6.4771

>AT73150 WP_006490972.1 NZ_CP019670:4506462-4506761 [Burkholderia cenocepacia]
MDKIKTRPWDSAEHLKTEDDMADYFDACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAAA

Nucleotide

• Length: 300 bp

>AT73150 NZ_CP019670:4506462-4506761 [Burkholderia cenocepacia]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
CTGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCCCGCGGCATGTCGC
AGGTCGCCCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCGTGA

Structures

Toxin

• AlphaFold DB: B1K4X0