Detailed information of TA system TA073148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 3647415..3648054
• Replicon: chromosome
• Accession: NZ_CP019670
• Organism: Burkholderia cenocepacia strain VC12802

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3N9F707
• Locus tag: A8E96_RS21945
• Protein ID: WP_011547639.1
• Coordinates: 3647644..3648054 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A8E96_RS21940
• Protein ID: WP_069231899.1
• Coordinates: 3647415..3647651 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E96_RS21920	3642786..3644174	-	1389	WP_077217309.1	DHA2 family efflux MFS transporter permease subunit	-
A8E96_RS34635	3644246..3644413	-	168	WP_155123349.1	hypothetical protein	-
A8E96_RS21925	3644423..3644644	-	222	WP_155123350.1	hypothetical protein	-
A8E96_RS21930	3645003..3645680	+	678	WP_077217310.1	hypothetical protein	-
A8E96_RS21935	3645700..3646926	-	1227	WP_077217290.1	acyltransferase	-
A8E96_RS21940	3647415..3647651	+	237	WP_069231899.1	DNA-binding protein	Antitoxin
A8E96_RS21945	3647644..3648054	+	411	WP_011547639.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8E96_RS21950	3648468..3648671	+	204	WP_006483056.1	cold-shock protein	-
A8E96_RS21955	3649133..3649396	+	264	WP_006483034.1	translation initiation factor IF-1	-
A8E96_RS21965	3649966..3651213	+	1248	WP_077217291.1	alkaline phosphatase family protein	-
A8E96_RS21970	3651210..3651458	+	249	WP_011547641.1	hypothetical protein	-
A8E96_RS21975	3651499..3652869	-	1371	WP_077217292.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15202.37 Da
• Isoelectric Point: 6.8816

>T73148 WP_011547639.1 NZ_CP019670:3647644-3648054 [Burkholderia cenocepacia]
MIDTNVISEIRKGRRTNRGVRAFFRQAEADASPLYLSVVTVAELRRGVDLIRHRGDHSQASALEAWIAMILSGYAPNILP
VDIETSQMWGHLRVPDPTHELDKLIAATALINDLTVVTRNVDDFARTGVRLLNPFD

Nucleotide

• Length: 411 bp

>T73148 NZ_CP019670:3647644-3648054 [Burkholderia cenocepacia]
TTGATTGATACCAACGTCATCAGCGAAATCAGAAAGGGCAGGCGAACCAATCGCGGCGTGCGGGCGTTCTTCAGGCAGGC
CGAAGCCGACGCGAGCCCGCTTTACCTGTCCGTCGTGACGGTGGCCGAGTTGCGGCGCGGCGTCGACCTGATCCGTCATC
GCGGCGATCATAGCCAGGCGTCGGCGCTCGAGGCCTGGATCGCGATGATCCTGTCGGGCTATGCGCCGAACATCCTGCCG
GTCGATATCGAGACCAGCCAGATGTGGGGGCATTTGCGGGTGCCCGATCCGACGCACGAACTCGACAAGCTGATCGCGGC
CACCGCGCTGATCAACGATCTGACGGTCGTCACGCGCAACGTCGACGATTTCGCTCGCACTGGCGTCCGGTTGCTGAATC
CGTTCGACTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8692.75 Da
• Isoelectric Point: 5.0916

>AT73148 WP_069231899.1 NZ_CP019670:3647415-3647651 [Burkholderia cenocepacia]
MANLLVRNVDDTLVQSLREQAAANGRSAEAEHRAILAEALGRPKRRTFAQVLLSMPEVGEDADFQRVQDSGEVRRVFD

Nucleotide

• Length: 237 bp

>AT73148 NZ_CP019670:3647415-3647651 [Burkholderia cenocepacia]
ATGGCAAATCTTCTGGTGCGTAACGTGGATGACACTCTCGTTCAAAGCCTGCGCGAGCAGGCGGCGGCTAATGGCAGGAG
CGCCGAGGCCGAACATCGAGCCATTCTGGCCGAAGCGCTCGGTCGGCCGAAGCGGCGGACATTTGCGCAAGTGCTGCTGA
GCATGCCGGAGGTGGGTGAGGACGCGGATTTCCAACGTGTTCAGGATTCAGGCGAGGTCAGGCGTGTTTTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A3N9F707