Detailed information of TA system TA073144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 2551617..2552254
• Replicon: chromosome
• Accession: NZ_CP019670
• Organism: Burkholderia cenocepacia strain VC12802

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8E96_RS17020
• Protein ID: WP_077220357.1
• Coordinates: 2551853..2552254 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B1JSY8
• Locus tag: A8E96_RS17015
• Protein ID: WP_012327630.1
• Coordinates: 2551617..2551853 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E96_RS17000	2547353..2548369	+	1017	WP_027807895.1	helix-turn-helix domain-containing protein	-
A8E96_RS17005	2548482..2549576	-	1095	WP_077220359.1	ADP-heptose--LPS heptosyltransferase	-
A8E96_RS17010	2549573..2551276	-	1704	WP_077220358.1	thiamine pyrophosphate-binding protein	-
A8E96_RS17015	2551617..2551853	+	237	WP_012327630.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A8E96_RS17020	2551853..2552254	+	402	WP_077220357.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8E96_RS17025	2552322..2553710	-	1389	WP_011546766.1	L-serine ammonia-lyase	-
A8E96_RS17030	2553741..2554883	-	1143	WP_012327633.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14957.04 Da
• Isoelectric Point: 5.5933

>T73144 WP_077220357.1 NZ_CP019670:2551853-2552254 [Burkholderia cenocepacia]
MPRFMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTACTSPARERHHLAALIEDIPVAPFDAAAAQA
YGPVRDATRERKKDHLDKLIAAHAVSLDVVLVTNNERDFASYPGVRLENWLND

Nucleotide

• Length: 402 bp

>T73144 NZ_CP019670:2551853-2552254 [Burkholderia cenocepacia]
ATGCCGCGCTTCATGCTCGACACCAACATGTGCATCTATTTGATGAAAAATCAGCCGGAGCAGGTCGCGAGACGATTTGC
GCAGTGCTACACGGGCGACGTCGTCATGTCGGCGATCACCTATGCGGAGCTCGAGTACGGCGTGACCGCATGCACGAGCC
CCGCCCGGGAGCGCCACCATCTCGCCGCATTGATCGAGGATATTCCCGTCGCGCCTTTCGACGCCGCGGCAGCCCAAGCG
TATGGCCCGGTGCGCGACGCAACCCGCGAGCGGAAGAAGGATCACCTCGACAAATTGATCGCCGCGCATGCGGTGTCGCT
CGATGTCGTCCTGGTGACCAACAACGAGCGGGATTTCGCGAGTTATCCGGGCGTGAGACTTGAGAACTGGTTGAACGACT
AG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9084.24 Da
• Isoelectric Point: 5.7316

>AT73144 WP_012327630.1 NZ_CP019670:2551617-2551853 [Burkholderia cenocepacia]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPMRRPLTQVLEKFAKFGPDFMTEGRGDHEQAEREGL

Nucleotide

• Length: 237 bp

>AT73144 NZ_CP019670:2551617-2551853 [Burkholderia cenocepacia]
ATGCATACCACGAGGGTATTCAGGAACGGCAACTCGCAGGCCGTGCGAATTCCTGCGGACCTTGCCTATGAGCGCAGCGA
TATCGAACTGGAAATCGAGCGCGTCGGTGACGAAATCCGCATTCGGCCGATGCGGCGGCCTTTGACTCAGGTACTGGAGA
AGTTCGCGAAATTCGGGCCTGACTTCATGACAGAAGGGCGTGGCGACCACGAGCAGGCCGAACGCGAGGGTTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A228I1F0