Detailed information of TA system TA073140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 7131208..7132434
• Replicon: chromosome
• Accession: NZ_CP019668
• Organism: Burkholderia cenocepacia strain VC7848

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A8E88_RS33445
• Protein ID: WP_077179322.1
• Coordinates: 7131208..7131789 (-)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: A8E88_RS33450
• Protein ID: WP_077179321.1
• Coordinates: 7131868..7132434 (-)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E88_RS33435	7127546..7129600	-	2055	WP_077179323.1	MacB family efflux pump subunit	-
A8E88_RS33440	7129603..7130796	-	1194	WP_069231467.1	efflux RND transporter periplasmic adaptor subunit	-
A8E88_RS35535	7131072..7131191	-	120	Protein_6391	SAM-dependent methyltransferase	-
A8E88_RS33445	7131208..7131789	-	582	WP_077179322.1	HD domain-containing protein	Toxin
A8E88_RS33450	7131868..7132434	-	567	WP_077179321.1	helix-turn-helix domain-containing protein	Antitoxin
A8E88_RS33455	7132873..7134276	+	1404	WP_077179320.1	hypothetical protein	-
A8E88_RS33460	7134376..7134954	+	579	WP_077179319.1	hypothetical protein	-
A8E88_RS33465	7135082..7135813	+	732	WP_027811744.1	ribonuclease T2	-
A8E88_RS33470	7135839..7136492	-	654	WP_077179318.1	cysteine hydrolase	-
A8E88_RS33475	7136732..7137352	+	621	WP_077179317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-172)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21306.98 Da
• Isoelectric Point: 5.5133

>T73140 WP_077179322.1 NZ_CP019668:c7131789-7131208 [Burkholderia cenocepacia]
MNPDTIHARLAFLREAERLKDVLRSGHTSAGRPESTAEHSWRLCLMALVFADALPGVDTLKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLMLTASLDRAVRDEIVALWDEYEAAASPEARAAKALDKLETIVQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFRAIREIVDADTQRKIDAGGHHA

Nucleotide

• Length: 582 bp

>T73140 NZ_CP019668:c7131789-7131208 [Burkholderia cenocepacia]
ATGAACCCCGATACGATCCACGCGCGACTCGCGTTCCTGCGCGAGGCCGAACGCCTGAAGGACGTGCTGCGCAGCGGCCA
TACGTCGGCCGGCCGTCCGGAAAGCACGGCCGAGCACAGCTGGCGGCTGTGCCTGATGGCGCTCGTGTTCGCCGACGCAC
TGCCCGGCGTCGATACGCTGAAGCTGCTGAAACTGTGCGTCGTCCACGACCTCGGCGAGGCGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCATCCCGACAAGAGCGCACATGAACGCGACGACCTGCTAATGCTGACCGCGTCGCTCGACCG
CGCGGTACGCGACGAGATCGTCGCGCTGTGGGACGAATACGAGGCGGCCGCATCGCCCGAAGCCCGCGCGGCGAAGGCAC
TCGACAAGCTCGAAACGATCGTGCAGCACAACCAGGGCAGCAATCCGCCCGATTTCGACTATGCGTTCAATCTCGGCTAC
GGCCGGCGCTATACCGACGCTGCGCCGCTGTTCCGCGCGATCCGCGAAATCGTCGACGCCGATACGCAGCGCAAGATCGA
CGCGGGTGGACACCACGCGTAA

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20532.43 Da
• Isoelectric Point: 11.0866

>AT73140 WP_077179321.1 NZ_CP019668:c7132434-7131868 [Burkholderia cenocepacia]
MPHPPLHRSSLGPQLKRWRALHRVKQSHAAELFGVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
SAGRMHLVCDLTHRLLASSPARAAEFSQPLSTLLGTSLWRYATPEIVRMEAALDPLGWHDRAGPPSVEFDTGANASRVVP
IRGSRCRWTRMTLSDGSAARLVETLDAH

Nucleotide

• Length: 567 bp

>AT73140 NZ_CP019668:c7132434-7131868 [Burkholderia cenocepacia]
ATGCCCCATCCGCCGCTTCATCGATCCTCGCTCGGCCCGCAACTCAAGCGCTGGCGCGCGCTGCACCGCGTGAAGCAAAG
CCATGCAGCCGAACTGTTCGGCGTCGCGCAATCGACGATCTCGCGCTGGGAAGCCGGCCTCCAGCAGATGTCGCCCGCCG
AGCGCGCGACGGCTGAACGGCTGCTCGCCGCTCGCCTCGATTCCGCGGGCGATCACGCACTCGCGCGGCTGATCGCCGGC
AGCGCGGGCCGCATGCATCTCGTGTGCGACCTCACGCACCGCCTGCTGGCCAGTTCACCGGCGCGCGCGGCCGAGTTCTC
GCAACCGCTTTCGACGTTGCTCGGCACGTCGCTGTGGCGCTACGCGACACCGGAAATCGTCCGCATGGAAGCCGCGCTCG
ATCCGCTCGGCTGGCACGACCGCGCGGGGCCGCCGAGCGTCGAGTTCGACACCGGCGCGAATGCGTCGCGCGTCGTGCCG
ATTCGCGGCAGCCGATGCCGGTGGACACGGATGACGCTGTCGGACGGCTCGGCCGCGCGGCTCGTGGAAACCCTCGACGC
ACACTGA