Detailed information of TA system TA073137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3823707..3824239
• Replicon: chromosome
• Accession: NZ_CP019668
• Organism: Burkholderia cenocepacia strain VC7848

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1JWG6
• Locus tag: A8E88_RS17970
• Protein ID: WP_011544674.1
• Coordinates: 3823916..3824239 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A8E88_RS17965
• Protein ID: WP_012327974.1
• Coordinates: 3823707..3823919 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E88_RS17930	3819193..3820488	+	1296	WP_059574261.1	Tol-Pal system protein TolB	-
A8E88_RS17935	3820506..3821018	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
A8E88_RS17940	3821038..3821787	+	750	WP_011544679.1	tol-pal system protein YbgF	-
A8E88_RS17950	3822191..3822511	-	321	WP_077178417.1	helix-turn-helix domain-containing protein	-
A8E88_RS17955	3822652..3823047	+	396	WP_077178416.1	hypothetical protein	-
A8E88_RS17960	3823115..3823594	+	480	WP_011694172.1	hypothetical protein	-
A8E88_RS17965	3823707..3823919	+	213	WP_012327974.1	antitoxin MazE family protein	Antitoxin
A8E88_RS17970	3823916..3824239	+	324	WP_011544674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A8E88_RS17975	3824559..3825083	+	525	WP_077178415.1	hypothetical protein	-
A8E88_RS17980	3825085..3826119	+	1035	WP_077178414.1	relaxase/mobilization nuclease domain-containing protein	-
A8E88_RS17985	3826242..3826685	-	444	WP_011544671.1	YiaA/YiaB family protein	-
A8E88_RS17990	3826789..3827736	-	948	WP_023476305.1	AraC family transcriptional regulator	-
A8E88_RS17995	3827866..3828195	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
A8E88_RS18000	3828394..3829227	+	834	WP_054927435.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11668.77 Da
• Isoelectric Point: 8.9366

>T73137 WP_011544674.1 NZ_CP019668:3823916-3824239 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSSMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T73137 NZ_CP019668:3823916-3824239 [Burkholderia cenocepacia]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGTGTAACGTTGCTGCTAATGTCGAGCTCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCGCAGATTGCCGTAGACAAAATGTTCACCGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTTGGGCTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7997.94 Da
• Isoelectric Point: 5.5492

>AT73137 WP_012327974.1 NZ_CP019668:3823707-3823919 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQATDLGHSQ

Nucleotide

• Length: 213 bp

>AT73137 NZ_CP019668:3823707-3823919 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCGGAGCGTGTTCGAATTCATCGCGAGAATCTTCGTGCGGCCGGGCTACGCCCAATTCAGATATG
GGTGCCCGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGATCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B1JWG6